Skip to main content
. Author manuscript; available in PMC: 2022 Sep 14.
Published in final edited form as: Immunity. 2021 Sep 14;54(9):2117–2132.e7. doi: 10.1016/j.immuni.2021.08.019

Figure 2. Melanoma/melanocyte Ag-specific memory T cells durably reside in lungs and lymph nodes.

Figure 2.

(A) Schematic diagram depicting parabiosis experiments involving mice with MAV (at least 30 days after tumor excision); CD8+ T cell populations were assessed in different tissues of donor vs. recipient mice either 14 days (B-D and F) or 30 days (E) after parabiotic joining. (B) Distribution of total CD45.1+ cells (representative mice gated on CD8+; left). Right: populations are further divided based on high/low expression of CD44. (C) Distribution of Thy1.1+ pmel cells (gated on CD8+ cells) across tissues. (D) Expression of CD103 and CD62L (gated on CD8+Thy1.1+ pmel cells. (E) Distribution of Thy1.1+ pmel cells (gated on CD8+ cells) after 30 days parabiotic joining. (F) Distribution of Thy1.1+ pmel cells in individual lymph nodes (gated on CD8+ cells). Symbols represent individual mice with lines joining parabiotic partners. Data in each panel are pooled from two independent experiments with the exception of panel (D), which depicts a single experiment. Each experiment was conducted twice with similar results. Significance was determined by paired t test (or by Wilcoxon matched pairs test for data that were not normally distributed); n.s. (non-significant) indicates p > 0.05.