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[Preprint]. 2022 Feb 7:2021.11.16.21266350. [Version 2] doi: 10.1101/2021.11.16.21266350

A booster dose of an inactivated SARS-CoV-2 vaccine increases neutralizing antibodies and T cells that recognize Delta and Omicron variants of concern

Bárbara M Schultz, Felipe Melo-González, Luisa F Duarte, Nicolás MS Gálvez, Gaspar A Pacheco, Jorge A Soto, Roslye V Berríos-Rojas, Liliana A González, Daniela Moreno-Tapia, Daniela Rivera-Pérez, Mariana Ríos, Yaneisi Vázquez, Guillermo Hoppe-Elsholz, Omar P Vallejos, Carolina Iturriaga, Marcela Urzua, María S Navarrete, Álvaro Rojas, Rodrigo Fasce, Jorge Fernández, Judith Mora, Eugenio Ramírez, Aracelly Gaete-Argel, Mónica Acevedo, Fernando Valiente-Echeverría, Ricardo Soto-Rifo, Daniela Weiskopf, Alba Grifoni, Alessandro Sette, Gang Zeng, Weining Meng; CoronaVac03CL Study Group, José V González-Aramundiz, Pablo A González, Katia Abarca, Alexis M Kalergis, Susan M Bueno
PMCID: PMC9016658  PMID: 35441179

Abstract

Background

CoronaVac ® is an inactivated SARS-CoV-2 vaccine approved by the World Health Organization. Previous studies reported increased levels of neutralizing antibodies and specific T cells two- and four-weeks after two doses of CoronaVac ® , but the levels of neutralizing antibodies are reduced at six to eight months after two doses. Here we report the effect of a booster dose of CoronaVac ® on the anti-SARS-CoV-2 immune response generated against variants of concern (VOC) Delta and Omicron in adults participating in a phase 3 clinical trial in Chile.

Methods

Volunteers immunized with two doses of CoronaVac ® in a four-week interval received a booster dose of the same vaccine between twenty-four and thirty weeks after the 2nd dose. Four weeks after the booster dose, neutralizing antibodies and T cell responses were measured. Neutralization capacities and T cell activation against VOC Delta and Omicron were detected at four weeks after the booster dose.

Findings

We observed a significant increase in neutralizing antibodies at four weeks after the booster dose. We also observed an increase in CD4 + T cells numbers over time, reaching a peak at four weeks after the booster dose. Furthermore, neutralizing antibodies and SARS-CoV-2 specific T cells induced by the booster showed activity against VOC Delta and Omicron.

Interpretation

Our results show that a booster dose of CoronaVac ® increases the anti-SARS-CoV-2 humoral and cellular immune responses in adults. Immunity induced by a booster dose of CoronaVac ® is active against VOC, suggesting an effective protection.

Full Text Availability

The license terms selected by the author(s) for this preprint version do not permit archiving in PMC. The full text is available from the preprint server.


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