Skip to main content
PMC home page
Springer Nature - PMC COVID-19 Collection logoLink to Springer Nature - PMC COVID-19 Collection
letter
. 2022 Apr 14;65(7):1473–1476. doi: 10.1007/s11427-021-2091-0

Metatranscriptomic analysis of host response and vaginal microbiome of patients with severe COVID-19

Meng Xiao 1,2,#, Bo Lu 3,4,#, Rui Ding 1,2, Xia Liu 5, Xian Wu 1,2, Yaqian Li 6, Xudong Liu 6, Lin Qiu 5, Zhibo Zhang 5, Jing Xie 7, Yu Chen 1,2, Dong Zhang 1,2, Liting Dong 3,4, Meiling Zhang 3, Jinying Peng 3, Hua Yang 5, Timothy Kudihna 8,9, Yingchun Xu 1,2, Taisheng Li 7, Chengqi Yi 3,4,✉, Lan Zhu 5,✉
PMCID: PMC9017960  PMID: 35441283

The content is available as a PDF (581.4 KB).

Electronic Supplementary Material

11427_2021_2091_MOESM1_ESM.pdf (4.2MB, pdf)

Methodology for TRACE-seq-based metatranscriptomic analysis of host response and microbiome in vagina of women

Acknowledgements

This work was supported by the National Key Research and Development Program of China (2020YFC0861000), Beijing Nova Program (Z201100006820127), International Innovation Resource Cooperation Project, Beijing Municipal Science and Technology Commission (Z201100008320024), the National Natural Science Foundation of China (31861143026, 91940304 and 21825701) and Epidemic Prevention and Control Special Project, Peking University. The funders played no role in study design, data collection, analysis, interpretation, report writing, or decision to submit the paper for publication. The authors would like to thank Vazyme Biotech in Nanjing, China, for assisting in library procedure optimization and providing library preparation kits. In addition, the authors would like to thank National Center for Protein Sciences at Peking University in Beijing, China, for assistance with experiments. Part of the analysis was performed on the High Performance Computing Platform of the Center for Life Science (Peking University).

Footnotes

Compliance and ethics

The authors have filed a patent application related to TRACE-seq (patent application number: 202011520074.X).

Contributed equally to this work

Contributor Information

Chengqi Yi, Email: chengqi.yi@pku.edu.cn.

Lan Zhu, Email: zhu_julie@vip.sina.com.

References

  1. Donders GGG, Bellen G, Grinceviciene S, Ruban K, Vieira-Baptista P. Aerobic vaginitis: no longer a stranger. Res Microbiol. 2017;168:845–858. doi: 10.1016/j.resmic.2017.04.004. [DOI] [PubMed] [Google Scholar]
  2. Fajgenbaum DC, June CH. Cytokine storm. N Engl J Med. 2020;383:2255–2273. doi: 10.1056/NEJMra2026131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ferrer M, Méndez-García C, Rojo D, Barbas C, Moya A. Antibiotic use and microbiome function. Biochem Pharmacol. 2017;134:114–126. doi: 10.1016/j.bcp.2016.09.007. [DOI] [PubMed] [Google Scholar]
  4. Gardner JK, Łaniewski P, Knight A, Haddad LB, Swaims-Kohlmeier A, Herbst-Kralovetz MM. Interleukin-36γ is elevated in cervicovaginal epithelial cells in women with bacterial vaginosis and in vitro after infection with microbes associated with bacterial vaginosis. J Infect Dis. 2020;221:983–988. doi: 10.1093/infdis/jiz514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gliniewicz K, Schneider GM, Ridenhour BJ, Williams CJ, Song Y, Farage MA, Miller K, Forney LJ. Comparison of the vaginal microbiomes of premenopausal and postmenopausal women. Front Microbiol. 2019;10:193. doi: 10.3389/fmicb.2019.00193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lu B, Dong L, Yi D, Zhang M, Zhu C, Li X, Yi C. Transposase-assisted tagmentation of RNA/DNA hybrid duplexes. eLife. 2020;9:e54919. doi: 10.7554/eLife.54919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ngo VL, Kuczma M, Maxim E, Denning TL. IL-36 cytokines and gut immunity. Immunology. 2021;163:145–154. doi: 10.1111/imm.13310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Park SY, Lee ES, Lee SR, Kim SH, Chae HD. Vaginal microbiome is associated with vulvodynia, vulvar pain syndrome: a case-control study. Sex Med. 2021;9:100314. doi: 10.1016/j.esxm.2020.100314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev. 2008;21:538–582. doi: 10.1128/CMR.00058-07. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shin D, Mukherjee R, Grewe D, Bojkova D, Baek K, Bhattacharya A, Schulz L, Widera M, Mehdipour AR, Tascher G, et al. Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Nature. 2020;587:657–662. doi: 10.1038/s41586-020-2601-5. [DOI] [PMC free article] [PubMed] [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

11427_2021_2091_MOESM1_ESM.pdf (4.2MB, pdf)

Methodology for TRACE-seq-based metatranscriptomic analysis of host response and microbiome in vagina of women

Articles from Science China. Life Sciences are provided here courtesy of Nature Publishing Group

RESOURCES