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(a) mRNA transcript levels of the neurotransmitters vGLUT1, GABA, TH, and aCHAT were assessed by qRT–PCR on days 0, 4, and 10. (b) Immunostaining labeling of neuron-like cells with the marker Syn1 on day 12 Scale bar, 50 μm. (c) Quantification of neuronal subtypes. (d) Immunostaining labeling of neurons-like cells with the neuronal markers MAP2, vGLUT1, GABA, and TH on day 15. Scale bar, 20 μm.
