Table 3.
Relative percentage (% rel.) of fatty acid methyl esters (FAME) from normal and defective CSB cells under hyperoxic, physioxic and hypoxic conditions 1.
| FAME | Normal CSB Cells | Defective CSB Cells | ||||
|---|---|---|---|---|---|---|
| Hyperoxia | Physioxia | Hypoxia | Hyperoxia | Physioxia | Hypoxia | |
| 14:0 | 0.89 ± 0.07 | 0.83 ± 0.16 | 0.84 ± 0.20 | 0.66 ± 0.10 | 0.55 ± 0.11 | 1.50 ± 0.43 |
| 16:0 | 20.37 ± 0.46 | 19.83 ± 0.20 | 25.48 ± 1.01 | 17.87 ± 0.33 | 18.07 ± 0.23 | 28.50 ± 0.91 |
| 18:0 | 14.87 ± 0.21 | 13.84 ± 0.31 | 15.06 ± 1.05 | 13.59 ± 0.46 | 16.05 ± 0.16 | 15.33 ± 0.41 |
| SFA | 36.13 ± 0.67 | 34.50 ± 0.18 | 41.38 ± 1.40 | 32.13 ± 0.47 | 34.67 ± 0.19 | 45.33 ± 1.13 |
| 6c-16:1 | 1.08 ± 0.11 | 1.26 ± 0.05 | 1.49 ± 0.30 | 1.12 ± 0.03 | 1.56 ± 0.09 | 2.15 ± 0.28 |
| 9c-16:1 | 4.19 ± 0.32 | 3.23 ± 0.16 | 2.66 ± 0.08 | 4.47 ± 0.09 | 1.82 ± 0.09 | 2.84 ± 1.15 |
| 8c-18:1 | 0.21 ± 0.03 | 0.66 ± 0.30 | 0.28 ± 0.03 | 0.17 ± 0.05 | 0.34 ± 0.02 | 0.49 ± 0.10 |
| 9c-18:1 | 25.35 ± 1.65 | 22.89 ± 0.32 | 25.68 ± 0.71 | 31.36 ± 0.76 | 22.05 ± 0.19 | 21.66 ± 2.99 |
| 11c-18:1 | 7.58 ± 0.84 | 8.36 ± 0.01 | 5.47 ± 0.23 | 9.28 ± 0.22 | 8.23 ± 0.10 | 5.30 ± 0.79 |
| 11c-20:1 | 0.28 ± 0.02 | 0.34 ± 0.04 | 0.20 ± 0.09 | 0.48 ± 0.01 | 0.42 ± 0.05 | 0.31 ± 0.14 |
| MUFA | 38.49 ± 2.69 | 36.74 ± 0.13 | 35.78 ± 0.60 | 46.71 ± 0.88 | 34.41 ± 0.29 | 32.75 ± 3.45 |
| 18:2-ω6 (LA) | 4.71 ± 0.31 | 6.10 ± 0.05 | 5.51 ± 0.44 | 4.19 ± 0.17 | 6.52 ± 0.02 | 5.27 ± 0.56 |
| 20:2 | 0.44 ± 0.00 | 0.49 ± 0.01 | 0.30 ± 0.10 | 0.52 ± 0.02 | 0.76 ± 0.02 | 0.35 ± 0.07 |
| 20:3 (DGLA) | 0.79 ± 0.02 | 0.56 ± 0.00 | 1.29 ± 0.13 | 1.06 ± 0.04 | 0.91 ± 0.03 | 1.32 ± 0.28 |
| 20:4 (ARA) | 12.42 ± 0.85 | 14.25 ± 0.09 | 8.94 ± 0.45 | 9.50 ± 0.59 | 16.02 ± 0.20 | 9.92 ± 2.84 |
| PUFA ω6 | 18.36 ± 1.14 | 21.78 ± 0.06 | 16.04 ± 0.64 | 15.27 ± 0.82 | 24.65 ± 0.25 | 16.87 ± 3.59 |
| 20:5 (EPA) | 0.46 ± 0.04 | 0.21 ± 0.02 | 0.25 ± 0.06 | 0.58 ± 0.05 | 0.37 ± 0.02 | 0.17 ± 0.04 |
| 22:5 (DPA) | 1.79 ± 0.32 | 2.12 ± 0.04 | 1.67 ± 0.16 | 1.19 ± 0.12 | 1.81 ± 0.02 | 1.44 ± 0.30 |
| 22:6 (DHA) | 3.34 ± 0.66 | 3.89 ± 0.03 | 3.88 ± 0.52 | 2.45 ± 0.27 | 3.23 ± 0.06 | 2.41 ± 0.58 |
| PUFA ω3 | 5.59 ± 1.01 | 6.22 ± 0.05 | 5.80 ± 0.51 | 4.22 ± 0.44 | 5.42 ± 0.06 | 4.02 ± 0.84 |
| 6t-16:1 | 0.09 ± 0.05 | 0.17 ± 0.01 | 0.12 ± 0.03 | 0.07 ± 0.06 | 0.18 ± 0.01 | 0.13 ± 0.01 |
| 9t-18:1 | 0.08 ± 0.04 | 0.10 ± 0.01 | 0.08 ± 0.02 | 0.20 ± 0.01 | 0.10 ± 0.01 | 0.10 ± 0.03 |
| mt 18:2-ω6 | 0.34 ± 0.01 | 0.16 ± 0.01 | 0.17 ± 0.05 | 0.36 ± 0.02 | 0.20 ± 0.01 | 0.12 ± 0.09 |
| mt 20:4-ω6 | 0.20 ± 0.01 | 0.23 ± 0.02 | 0.13 ± 0.03 | 0.18 ± 0.02 | 0.24 ± 0.03 | 0.11 ± 0.01 |
| TFA | 0.70 ± 0.09 | 0.65 ± 0.05 | 0.51 ± 0.06 | 0.81 ± 0.05 | 0.71 ± 0.05 | 0.45 ± 0.11 |
| 5c,8c-18:2 | 0.53 ± 0.11 | 0.50 ± 0.05 | 0.40 ± 0.07 | 0.69 ± 0.10 | 0.58 ± 0.10 | 0.51 ± 0.06 |
1 The values are obtained from the GC analysis of FAME, derived from cell membrane phospholipids as reported in the experimental part. All measurements were performed in triplicate per group and the values are given as mean ± SD. For a graphical presentation of FAME, see Figure S2.