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. 2022 Apr 17;14(4):833. doi: 10.3390/v14040833

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Establishment of one-tube multiplex LAMP-Cas12a assay for detection of four porcine diarrhea coronaviruses. (A) Schematic diagram experimental flow and principle of contamination-free one-tube multiplex LAMP-Cas12a assay. (B) Colorimetric/fluorescence readout of four porcine coronaviruses by one-tube multiplex LAMP-Cas12a assay. 1: plasmid DNA mixture of PEDV, TGEV, PDCoV, and SADS-CoV (1:1:1:1) as templates; 2: non-template control (NTC). sgRNA, small guide RNA; LAMP, loop-mediated isothermal amplification; ROX-BHQ2, ROX-labeled ssDNA-FQ reporter; No excitation light stands for white light; PEDV, porcine epidemic diarrhea virus; TGEV, transmissible gastroenteritis virus; PDCoV, porcine deltacoronavirus; SADS-CoV, swine acute diarrhea syndrome coronavirus.