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HIV infection of 3D organoids. (A) Three-dimensional cerebral organoids, starting from week 5 up to week 9, were infected with 100 ng (p24 Gag) HIVbal. Cerebral organoids were derived from iPSC line OH1.5 and OH2.6. (B) Matrigel droplets were infected with 10 ng and 100 ng (p24 Gag) HIVbal. Supernatant was collected at each timepoint and analyzed for luciferase activity. The means ± standard errors of the means are shown.
