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. 2022 Apr 8;11(8):1262. doi: 10.3390/cells11081262

Figure 3.

Figure 3

SMYD2 inhibition decreases TMPRSS2 expression in Caco-2 cells. (A,B) LDH release assay from Caco-2 cells treated with increasing concentrations of AZ505, AZ506, LLY507, or BAY598 for 24 h (A) and 48 h (B). Experiments were performed two times and representative data are shown. *** p < 0.001. N.S., not significant. (C) Immunofluorescence staining of TMPRSS2 in Caco-2 cells stimulated with vehicle (mock), 10 µM AZ505, 10 µM AZ506, 10 µM LLY507, or 20 µM BAY598 for 8 h. Scale bar, 10 µm. (D) Immunoblot analysis of TMPRSS2 in lysates of Caco-2 cells stimulated with 20 µM BAY598 at different time points. β-actin served as a loading control. (E) Densitometric analysis of TMPRSS2 expression.