Skip to main content
. 2022 Apr 8;11(8):1262. doi: 10.3390/cells11081262

Figure 4.

Figure 4

SMYD2 inhibition decreases SARS-CoV-2 infection in Caco-2 cells. (A,B) Antiviral effect of AZ505 (A) and BAY598 (B) in Caco-2 cells. Cells were treated with the indicated concentrations for 24 h prior to infection. The same amount of inhibitor was also applied during infection (MOI = 0.005). SARS-CoV-2 positive cells were visualized two days post-infection using a mAb against Spike and an Alexa488-conjugated secondary antibody under a Nikon Inverted Ts2-FL Eclipse microscope equipped with a 10x objective. The Alexa488 signal was quantified in a Victor X4 multilabel reader. Bar graphs represent mean values of eight infected wells ± SD. *** p < 0.001. N.S., not significant. One representative experiment out of three replicates is depicted. (C) Plaque assay of Caco-2 cells infected with a recombinant SARS-CoV-2 reporter virus expressing EYFP instead of the viral ORF6 gene (1:10,000 dilution) after treatment with the indicated concentrations of BAY598. EYFP-positive plaques were detected with a fluorescence imager two days post-infection. The experiment was repeated three times and representative results are shown.