Figure 6.

SMYD2 inhibition does not affect SARS-CoV-2 infection of Vero E6 cells. (A,B) Antiviral effect of AZ505 and BAY598 in Vero E6 cells. Cells were treated with the indicated concentrations for 24 h prior to infection. The same amount of inhibitor was also applied together with the virus (MOI = 0.005). SARS-CoV-2 positive cells were visualized two days post-infection using a mAb against Spike and an Alexa488-conjugated secondary antibody under a Nikon Inverted Ts2-FL Eclipse microscope equipped with a 10× objective. The Alexa488 signal was quantified in a Victor X4 multilabel reader. Bar graphs represent mean values of eight infected wells ± SD. N.S., not significant. One representative experiment out of three replicates is shown.