REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-ZO1 tight junction protein rabbit polyclonal	Servicebio	Cat# GB111402; RRID:AB_2910249
Anti-MMP2 rabbit polyclonal	Servicebio	Cat# GB11130; RRID:AB_2910250
Anti-Cytokeratin 3/CK-3 mouse monoclonal	Abcam	Abcam Cat# ab68260; RRID:AB_1140695
Cy3 conjugated Goat Anti-Rabbit IgG (H + L)	Servicebio	Cat# GB21303; RRID:AB_2861435
FITC conjugated Goat Anti-Rabbit IgG (H + L)	Servicebio	Cat# GB22303; RRID:AB_2904189
Cy5 conjugated Goat Anti-Mouse IgG (H + L)	Servicebio	GB27301; RRID:AB_2910251
Chemicals, peptides, and recombinant proteins
Polydimethylsiloxane	DOWCORNING	SYLGARDTM 184
SU-8	MicroChem	2100
Fetal bovine serum	Gibco	10099–141C
Human EGF	Peprotech	AF-100-15-100
Penicillin-streptomycin	Hyclone	SV30010
Recombinant human insulin	Applied Biological Materials Inc.	Z101065
Transferrin human	Sigma-Aldrich	T8158
Sodium selenite	Sigma-Aldrich	S5261
Hydrocortisone	Selleck Chemicals	S1696
β-estradiol	Sigma-Aldrich	E2758
Recombinant human VEGF (165aa)	Applied Biological Materials Inc.	Z100895
Heparin sodium	Selleck Chemicals	S1346
L-glutamine	Applied Biological Materials Inc.	G275
Collagen I, Rat Tail	Corning Inc.	354249
Paraformaldehyde	Sangon	E672002-0100l
Hematoxylin	Servicebio	G1005-1
Eosin	Servicebio	G1005-2
4′,6′-diamidino-2-phenylindole hydrochloride	Invitrogen	D1306
Fluorescein isothiocyanate–dextran (average MW 3000–5000)	Aladdin	F121152
Critical commercial assays
Calcein-AM/PI double stain kit	Dojindo	C542
Cell Counting Kit-8 assay	Beyotime Biotechnology	C0043
Mesenchymal stem cell growth medium kit	Cyagen	HUXMF-90011
Matrix metallopeptidase 2 protein ELISA kit	Beyotime Biotechnology	PM730
Experimental models: Cell lines
Immortalized human corneal epithelial cells	RIKEN Biosource Center	N/A
Immortalized HCEnd cells	Applied Biological Materials Inc.	T5077
Bone marrow-derived mesenchymal stem cells	Cyagen	HUXMF-01001
Software and algorithms
Image Pro Plus	Media Cybernetic Inc.	6.0
GraphPad Prism	GraphPad Software Inc.	Prism 8
MATLAB	MathWorks	R2019a