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. 2021 Jun 8;18(3):518–539. doi: 10.1080/15548627.2021.1936356

Figure 5.

Figure 5.

Effect of G. cambogia extract on KLF3 expression in 3T3-L1 preadipocytes during differentiation. (A) Effect of G. cambogia extract (300 μg/ml) on KLF3, CEBPA and PPARG in 3T3-L1 differentiated cells for the indicated times (n = 4 per group). **p < 0.01 vs. each control. (B) Effect of G. cambogia extract (0–300 μg/ml) on CEBPB, KLF3, CEBPA and PPARG expression in 3T3-L1 differentiated cells after 72 h (n = 4 per group). *p < 0.05 and **p < 0.01 vs. Con. (C) Effect of FMK (3 μM) and stattic (5 μM) on CEBPB, KLF3, CEBPA and PPARG expression in 3T3-L1 differentiated cells for the indicated times (n = 4 per group). *p < 0.05 and **p < 0.01 vs. each control. (D) Effect of Cebpb, Rps6ka1 and Stat3 knockdown on KLF3 expression in 3T3-L1 differentiated cells after 72 h (n = 4 per group). **p < 0.01 vs. siCon. n.s.: not significant. (E) Effect of G. cambogia extract (300 μg/ml) on Klf3 transcript levels in 3T3-L1 differentiated cells for the indicated times (n = 9 per group). (F) Effect of G. cambogia extract (300 μg/ml) on the KLF3 protein half-life in 3T3-L1 differentiated cells after 72 h. After treatment of G. cambogia extract in cells, cycloheximide (CHX, 1.5 μg/ml) was coincubated with the indicated times (n = 4 per group). **p < 0.01 vs. each control. (G) Effect of G. cambogia extract (Ga, 300 μg/ml) for 72 h and MG132 (10 μM) and 3-MA (0.5 mM) for 24 h on SQSTM1 and KLF3 expression in 3T3-L1 differentiated cells (n = 4 per group). **p < 0.01 vs. Con. The data are the mean ± S.D.