FIG 5.

Genotype-dependent effect of phosphate depletion on root lipids, microbiota, and plant biomass. (a) CPCoA based on the Euclidean distance of the root lipid profiles of the wild type (WT) and the P transporter-defective line pht1;6 at both growth stages (71 lipid compounds were analyzed [n = 41] [data can be found at GitHub]). (b) CPCoA based on Bray-Curtis dissimilarity of the root bacterial and fungal communities (n = 48 for bacterial and fungal communities) and constrained by plant genotype, plant growth stage, and soil management (depletion/amendment of P on soil). (c) Effects of genotype, plant growth stage, and P fertilization on microbial community alpha-diversity (n = 6 per plot) (V, vegetative stage; R, reproductive stage). (d) Effects of genotype, plant growth stage, and P fertilization on four plant biomass parameters (n = 43). A Wilcoxon test followed by FDR correction was used for statistical analysis (P < 0.05). Capital letters indicate significant differences between growth stages; lowercase letters indicate significant differences between genotypes in specific soil management, within each growth stage. ns, not significant.