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. 2022 Mar 7;13(2):e03458-21. doi: 10.1128/mbio.03458-21

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Copyright © 2022 Matilla et al.

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FIG 6 — Isothermal titration calorimetry study of the binding of different ligands to the LBD of the P. atrosepticum SCRI1043 chemoreceptor ECA_RS10935 (PacA). (Upper panel) Raw data for the titration of 20 μM protein with 9.6-μL aliquots of 1 mM (betaine, carnitine) and 5 mM (acetylcholine) ligand solutions. (Lower panel) Concentration-normalized and dilution heat-corrected raw data for the titration with acetylcholine and choline. The continuous line is the best fit with the “one binding site model” of the MicroCal version of ORIGIN.