Figure 7.

Manipulating the material properties of oskar granules interferes with oskar anchoring and translation and impairs embryonic development

(A) Stage 10b egg chamber expressing MCP-2xEGFP or MCP-EGFP-FUS LC in oskar RNA-null background (osk^A87/Df3Rp^XT103).

(B) Fusion of FUS LC-oskar granules (grayscale) at the posterior of stage 10b egg chambers.

(C) Immunostaining and quantification of Oskar protein (magenta) intensity in oskar RNA-null stage 10b egg chambers. The genotype in the fourth panel is osk⁸⁴/Df3Rp^XT103 where the anchoring function is provided in trans. All images shown and quantified were acquired using identical microscope settings and representations contrast matched. Error bars, SD; n, number of analyzed oocytes. Unpaired Student’s t test used for comparisons. Significance level: ^∗∗∗∗ < 0.0001.

(D) Pole cells identified by Vasa immunostaining (magenta) and nuclei stained with DAPI (blue) in embryos of the indicated genotypes.

(E) Pattern of even skipped (Eve) stripes (green) and representative cuticles of embryos of the indicated genotypes; anterior to the left, ventral to the bottom. t1–t3, thoracic segments; a1–a8, abdominal segments; cs, head skeleton (black arrow); fk, filzkörper (white arrow); black arrowhead, patchy band of denticles. Refer to Figure S8H for phenotypic classes observed.

(F) Quantification of hatching rate of eggs of the indicated genotypes. Error bars, SD; n, number of eggs scored. See also Figure S8; Video S6.