REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat Anti-Monkey IgG H&L (HRP)	Abcam	Cat#ab112767; RRID:AB_10866625
CB6	Shi et al., 2020	N/A
CV07-270	Kreye et al., 2020	N/A
C110	Barnes et al., 2020	N/A
S309	Pinto et al., 2020	N/A
CR3022	Yuan et al., 2020	N/A
Bacterial and virus strains
Prototype SARS-CoV-2, hCoV-19/China/CAS-B001/2020 strain	Isolated from a SARS-CoV-2 infected patient by IMCAS, China	GISAID: EPI_ISL_514256-7
Prototype SARS-CoV-2, IVDC-QD-11-2P2 strain	Isolated from a SARS-CoV-2 infected patient by China CDC	N/A
Prototype SARS-CoV-2, GDPCC-nCoV27 strain	Imported from Guangdong CDC, China	N/A
Alpha variant SARS-CoV-2, SARS-CoV-2/C-Tan-BJ202101(B1.1.7) strain	Isolated from a SARS-CoV-2 infected patient by China CDC	NPRC: 2. 062100002
Beta variant SARS-CoV-2, GDPCC-nCoV84 strain	Imported from Guangdong CDC, China	NPRC: 2.062100001
Delta variant SARS-CoV-2, CCPM-B-V-049-2105-8 strain	Imported from Chongqing CDC, China	N/A
Omicron (BA.1) variant SARS-CoV-2	Isolated from a SARS-CoV-2 infected patient by China CDC	NPRC: 2.192100005
Omicron (BA.1) variant SARS-CoV-2, CCPM-B-V-049-2112-18 strain	Imported from Institute of Laboratory Animals Science, CAMS & PUMC, China	N/A
SARS-CoV-2 pseudovirus	This paper	N/A
Ad5-hACE2	Provided by Gary Wong, Institut Pasteur of Shanghai, CAS, China	N/A
Biological samples
Serum samples from BALB/c mice	This paper	N/A
Serum samples from rhesus macaques	This paper	N/A
Chemicals, peptides, and recombinant proteins
AddaVax adjuvant	InvivoGen	Cat#vac-adx-10
Prototype SARS-CoV-2 RBD peptide pool	Beijing SciLight Biotechnology Ltd. Co.	N/A
Recombinant prototype SARS-CoV-2-S protein RBD monomer, spike residues 319-541, GenBank: YP_009724390	Dai et al., 2020	N/A
Recombinant Beta variant SARS-CoV-2-S protein RBD monomer, spike residues 319-541, GISAID: EPI_ISL_736940	This paper	N/A
Recombinant Delta variant SARS-CoV-2-S protein RBD monomer, spike residues 319-541, GenBank: OK091006.1	This paper	N/A
Recombinant Omicron (BA.1) variant SARS-CoV-2-S protein RBD monomer, spike residues 316-534, GISAID: EPI_ISL_6795848	ACROBiosystems	Cat#SPD-C522e
Recombinant prototype SARS-CoV-2-S protein RBD-dimer, spike residues 319-537, two copies in tandem, GenBank: YP_009724390	Dai et al., 2020	N/A
Recombinant Beta variant SARS-CoV-2-S protein RBD-dimer, spike residues 319-537, two copies in tandem, GISAID: EPI_ISL_736940	This paper	N/A
Recombinant prototype-Beta chimeric RBD-dimer, spike residues 319-537 (prototype, GenBank: YP_009724390) and 319-537 (Beta variant, GISAID: EPI_ISL_736940)	This paper	N/A
GMP grade of recombinant prototype-Beta chimeric RBD-dimer, spike residues 319-537 (prototype, GenBank: YP_009724390) and 320-537 (Beta variant, GISAID: EPI_ISL_736940)	Anhui Zhifei Longcom Biopharmaceutical Co. Ltd	N/A
Recombinant Delta-Omicon chimeric RBD-dimer, spike residues 319-537 (Delta variant, GenBank: OK091006.1) and 316-534 (Omicron variant, GISAID: EPI_ISL_6795848)	This paper	N/A
Recombinant hACE2 protein, residues 1-740	Sino Biological Inc.	Cat#10108-H08H
Critical commercial assays
HisTrap HP 5 mL column	GE Healthcare	Cat#17524802
HiLoad™ 16/600 Superdex™ 200 pg column	GE Healthcare	Cat#28989335
Superdex™ 200 Increase 10/300 GL column	GE Healthcare	Cat#28990944
HiTrap Protein A HP	Cytiva	Cat#17040303
Series S Sensor Chip CM5	GE Healthcare	Cat# 29-1496-03
Trizol	Thermo Fisher Scientific	Cat# 10296028
FastKing One Step Probe RT-qPCR kit	Tiangen Biotech	Cat#FP314
TaqMan Fast Virus 1-Step Master Mix kit	Thermo Fisher Scientific	Cat#4444434
Monkey INF-γ ELISpotPLUS kit (ALP)	MabTech	Cat#3421M-4APW-10
Monkey IL-2 ELISpotPLUS kit (ALP)	MabTech	Cat#3445M-4APW-10
Human IL-4 ELISpotPRO kit (ALP)	MabTech	Cat#3410-2APW-10
Deposited data
Cryo-EM structure of SARS-CoV-2 prototype RBD-dimer bound to CB6 Fab	This paper	EMDB:EMD-33225
Cryo-EM structure of SARS-CoV-2 prototype-Beta chimeric RBD-dimer bound to CB6 Fab	This paper	EMDB:EMD-33234
Cryo-EM structure of SARS-CoV-2 Delta-Omicron chimeric RBD-dimer bound to CB6 Fab	This paper	EMDB:EMD-33235
Experimental models: Cell lines
Expi293F Cells	Thermo Fisher Scientific	Cat#A14527; RRID:CVCL_D615
Vero cells	ATCC	CCL-81; RRID:CVCL_0059
Vero-E6 cells	ATCC	CRL-1586; RRID:CVCL_0574
CHOZN® CHO K1 host cell line	SAFC	Cat#CHOK1-1VL
Experimental models: Organisms/strains
BALB/c mice	Beijing Vital River Laboratory Animal Technology Co., Ltd. (licensed by Charles River)	N/A
Rhesus macaques	Qingwei Macaque Breeding Centre, Chengkou County, Chongqing, China	N/A
Oligonucleotides
gRNA-F, GACCCCAAAATCAGCGAAAT	GENEWIZ and Thermo Fisher Scientific	N/A
gRNA-R, TCTGGTTACTGCCAGTTGAATCTG	GENEWIZ and Thermo Fisher Scientific	N/A
gRNA-probe, ACCCCGCATTACGTTTGGTGGACC	GENEWIZ and Thermo Fisher Scientific	N/A
sgRNA-F, CGATCTCTTGTAGATCTGTTCTC	GENEWIZ and Thermo Fisher Scientific	N/A
sgRNA-R, ATATTGCAGCAGTACGCACACA	GENEWIZ and Thermo Fisher Scientific	N/A
sgRNA-probe, ACACTAGCCATCCTTACTGCGCTTCG	GENEWIZ and Thermo Fisher Scientific	N/A
gRNA-Omicron-probe, ACTCCGCATTACGTTTGGTGGACC	Thermo Fisher Scientific	N/A
Recombinant DNA
pCAGGS	MiaoLingPlasmid	N/A
pCAGGS_prototype_SARS-CoV-2_RBD_monomer, residues 319-541, GenBank: YP_009724390	Dai et al., 2020	N/A
pCAGGS_Beta_variant_SARS-CoV-2_RBD_monomer, residues 319-541, GISAID: EPI_ISL_736940	This paper	N/A
pCAGGS_Delta_variant_SARS-CoV-2_RBD monomer, residues 319-541, GenBank: OK091006.1	This paper	N/A
pCAGGS_prototype_SARS-CoV-2_RBD-dimer, residues 319-537, two copies in tandem, GenBank: YP_009724390	Dai et al., 2020	N/A
pCAGGS_Beta_variant_SARS-CoV-2_RBD-dimer, residues 319-537, two copies in tandem, GISAID: EPI_ISL_736940	This paper	N/A
pCAGGS_prototype-Beta_chimeric_RBD-dimer, residues 319-537 (prototype, GenBank: YP_009724390) and 319-537 (Beta variant, GISAID: EPI_ISL_736940)	This paper	N/A
pCAGGS_Delta-Omicon_chimeric_RBD-dimer, residues 319-537 (Delta variant, GenBank: OK091006.1) and 316-534 (Omicron variant, GISAID: EPI_ISL_6795848)	This paper	N/A
pCAGGS_CB6 Fab light chain	This paper	N/A
pCAGGS_CB6 Fab heavy chain	This paper	N/A
pCAGGS_CV07-270 light chain	This paper	N/A
pCAGGS_CV07-270 heavy chain	This paper	N/A
pCAGGS_C110 light chain	This paper	N/A
pCAGGS_C110 heavy chain	This paper	N/A
pCAGGS_S309 light chain	This paper	N/A
pCAGGS_S309 heavy chain	This paper	N/A
pCAGGS_CR3022 light chain	This paper	N/A
pCAGGS_CR3022 heavy chain	This paper	N/A
Software and algorithms
MotionCor2	Zheng et al., 2017	https://emcore.ucsf.edu/ucsf-motioncor2
CTFFIND4.1	Rohou and Grigorieff, 2015	N/A
RELION3.1	Zivanov et al., 2018	http://www2.mrc-lmb.cam.ac.uk/relion
Chimera	Pettersen et al., 2004	http://www.cgl.ucsf.edu/chimera
CryoSPARC	Punjani et al., 2017	https://cryosparc.com/
BIAevaluation Version 3.0	GE Healthcare	N/A
GraphPad Prism	GraphPad Software	https://www.graphpad.com/
OriginPro 2018	OriginLab Cororation	http://www.OriginLab.com
Other
BIAcore 8000	GE Healthcare	N/A
Vitrobot Mark IV	Thermo Fisher Scientifific	N/A
Quantifoil R 1.2/1.3 holey carbon grids	Quantifoil	N/A
Titan Krios microscope	Thermo Fisher Scientifific	N/A