FIG 1.
Establishment of reproducible C. auris infection in immunocompetent C57BL/6 mice. Eight-week-old, immunocompetent female C57BL/6 mice were intravenously injected with approximately 1.5 × 108, 4 × 108, or 8 × 108 yeast cells of C. auris isolates AR-0381 or AR-0384. For each strain, two independent experiments were performed. Aggregated results are shown. (A) Survival curves were compared using the log rank test. (B) Distributions of 7-day murine sepsis scores (MSS) were used as a measure of infection severity. Black bars indicate medians among all mice. Thin colored bars indicate medians among survivors. (C) Numbers of C. auris CFU were quantified by streaking kidney tissue homogenates on Sabouraud dextrose agar upon natural death or on day 7 postinfection. Black bars = medians. (B and C) Kruskal-Wallis test with Dunn’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.