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. 2022 Apr 20;604(7907):749–756. doi: 10.1038/s41586-022-04638-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a,b, heat map (a) and gene set enrichment analysis (GSEA) (b) of differential gene expression in FT282 parental (WT) vs CCNE1-high (C3 and C4 for heat map, C4 for GSEA) cells for a gene set comprising genes co-regulated by MMB-FOXM1. Development of the co-regulated MMB-FOXM1 gene list is available in Methods and the list of genes is available in Extended Data Table 3. c, Clonogenic survival of CCNE1-high FT282-hTERT TP53^R175H cells nucleofected with Cas9 ribonucleoproteins assembled with the indicated sgRNAs. Shown on top are representative images of plates stained with crystal violet and below is the quantitation. Data are shown as mean ± s.d. (n = 3). d, QIBC quantitation of cells with pan-γH2AX as a function of RP-6306 dose of CCNE1-high FT282-hTERT TP53^R175H cells nucleofected with Cas9 ribonucleoproteins assembled with the indicated sgRNAs. Data are shown as mean ± s.d. (n = 3). e,f, Whole cell lysates of FT282-hTERT TP53^R175H parental and MYBL2-2A-GFP expressing (e) and FT282-hTERT TP53^R175H MYBL2-2A-GFP WT and CCNE1-high cells (f, left) were immunoblotted with MYBL2, MYBL2-pT487 and Tubulin specific antibodies. (f, left) * and ** denote the lower and upper phosphorylated MYBL2 bands respectively. (f, right) Quantitation of upper to lower band intensity. Data are shown as mean ± s.d. (n = 3). (e) Representative of two immunoblots. For gel source data, see Supplementary Fig. 1.

Source data