Electrospun silk fibroin/TiO₂ mats. Preparation, characterization and efficiency for the photocatalytic solar treatment of pesticide polluted water

Abstract

The photocatalytic properties of silk fibroin (SF) incorporating TiO₂ nanoparticles using an electrospinning technique were examined. Electrospun SF/TiO₂ mats were successfully prepared and characterized by different techniques (XRD, FE-SEM, XPS, XDS, FTIR and BET). The photocatalytic efficiency of these materials were assessed by their ability to degrade four pesticides (boscalid, hexythiazox, pyraclostrobin and trifloxystrobin) in water exposed to solar irradiation. The effect of catalyst loading on the disappearance kinetics of the different pesticides was studied in order to determine the maximum degradation efficiency. The degradation rate significantly increases upon adding the TiO₂. However, no significant differences (p < 0.05) were observed when the TiO₂ loading was increased from 25 to 50 mg for most compounds. Thus, SF mats with 25 mg of TiO₂ were selected. Therefore, a new and simple approach to produce materials with photocatalytic activity, safety and potential application in the purification of water contaminated by pesticides has been developed.

The photocatalytic properties of silk fibroin (SF) incorporating TiO₂ nanoparticles using an electrospinning technique were examined.

1. Introduction

Water pollution by organic compounds, such as pesticides, is one of the most complex environmental problems faced by researchers. Conventional biological and physical treatments of water are neither effective nor sustainable for the most recalcitrant pesticides. In this context, the development of solar photochemical technologies whereby sunlight is absorbed by a catalyst in order to change the structure of pesticides has recently aroused great interest among the scientific community for water treatment. TiO₂ nanoparticles are described in the scientific literature as efficient photocatalysts,¹ used for the degradation of a wide variety of organic pollutants due to their high activity and splendid electronic and optical properties.^2–4 In this context many attempts have been made to incorporate them into three-dimensional supports⁵ that minimize the risks inherent in their systemic use for the environment and drawbacks, such as a stage of separation and recovery of TiO₂ nanoparticles.

Electrospinning is a procedure used to make micro- and nanofibrous scaffolds with uses in a diverse fields such as purification, catalysts,⁶ electrical devices and biomedicine.^7–9 Silk fibroin (SF) produced by the silkworm (Bombyx mori) is a biocompatible¹⁰ and natural protein that has been extensively used in recent years to produce electrospun scaffolds with a diversity of applications.¹¹ The structural unit of SF is composed of three elements, the heavy chain fibroin (350 kDa), the light chain fibroin (25 kDa) and protein P25, the molar proportion of these constituents is 6 : 6 : 1 respectively.¹² Additionally, SF electrospinning is commonly combined with additional functionalizing methods. Such as those involving the incorporation of molecules of interest (growth factors among others),¹³ the utilization of different SF blends in combination with other polymers,¹⁴ the adsorption of conductive polymers on its surface^15,16 or even the combination with nanoparticles designed to catalyze the degradation of chemical warfare agents.⁶

Several research groups have also combined sericulture and TiO₂ nanoparticles in works of diverse nature. In this way, some authors have even fed silkworm larvae with TiO₂ nanoparticles in different concentrations stating improvements in terms of growth, feed efficiency and silk production.¹⁷ Other researchers have described improvements in the reproductive efficiency of B. mori with an increase in size, quantity and fertility of the eggs.¹⁸ Cai et al.¹⁹ also described the production of silk fibres more resistant to UV light and with reinforced mechanical properties using the same feeding technique. Furthermore, some authors have used regenerated fibroin by combining it with TiO₂ nanoparticles with different formats and applications, such as films²⁰ or porous scaffolds²¹ for tissue engineering with improved mechanical and thermal properties. In relation to the field of electrospinning, the manufacture of silk fibroin/TiO₂ nanofibrous mats for wound dressings has also been explored, using formic acid as a solvent.²² Another studied approach has been the electrospinning of PVA/fibroin/TiO₂ mixtures to produce a photocatalytic textile.²³ However, to the best of our knowledge, electrospun SF/TiO₂ mats have never used as photocatalyst for the removal of pesticides in water.

SF has optimal properties in terms of handling, safety and biocompatibility. Additionally, the potential manufacture of materials incorporating TiO₂ nanoparticles and SF in their composition is very promising. In this view, the aim of this work was to develop a simple, effective and innovative way to produce these kind of materials by means of electrospinning of SF dissolutions in combination with several rounds of atomization of methanolic suspensions of TiO₂ nanoparticles. In addition, the photocatalytic efficiency of these materials were also assessed by their ability to degrade four pesticides (boscalid, pyraclostrobin, hexythiazox and trifloxystrobin) in water exposed to solar irradiation.

2. Materials and methods

2.1. Insecticides and reagents

Analytical standards (boscalid, pyraclostrobin, hexythiazox and trifloxystrobin) with a purity > 99% were supplied from Dr Ehrenstorfer (Augsburg, Germany). Three commercial pesticides, Signum 26.7% w/w (boscalid) +6.7% w/w (pyraclostrobin), Jalisco 10% w/v (hexythiazox) and Flint 50% w/w (trifloxystrobin) were used for the photodegradation experiments. The main physicochemical properties of the studied insecticides are summarized in Table 1. The commercial titanium dioxide P25 (99.5%, <21 nm, 50 m² g⁻¹) was purchased from Nippon Aerosil Co Ltd (Osaka, Japan). Acetonitrile was purchased from Scharlau (Barcelona, Spain). A Milli-RX Water Purification System (Millipore, Bedford, MA, USA) was used to obtain deionized water.

Physical–chemical characteristics of the pesticides used in this study.

Compound	MFa	M W b	log KOWc	VPd	WSe	GUSf
Boscalid	C18H12Cl2N2O	343.2	2.96	7.2 × 10−4	4.6	2.66
Pyraclostrobin	C19H18BrClN3O4	387.8	3.99	2.6 × 10−5	1.9	0.06
Hexythiazox	C17H21ClN2O2S	352.9	2.67	1.3 × 10−3	0.5	0.03
Trifloxystrobin	C20H19F3N2O4	408.4	4.50	3.4 × 10−4	0.6	0.19

^aMolecular formula.

^bMolecular mass (g mol⁻¹).

^cOctanol/water partition coefficient (K_OW).

^dVapour pressure (mPa at 25 °C).

^eWater solubility (mg L⁻¹).

^fGUS leaching potential index.

2.2. Silk fibroin processing

Cocoons of B. mori were produced in the facilities of the IMIDA (Murcia, Spain). After chopping them into 4 pieces, they were boiled in 0.02 M Na₂CO₃ for 30 min, in order to perform the removal of the sericin (also known as degumming step). Then, the fresh SF was carefully washed with water and dehydrated at room temperature during 3 days. SF was subsequently dissolved using 9.3 M LiBr (Acros Organics) as a solvent for 3 h (60 °C), giving rise to a 20 wt% dissolution. Then it was dialysed against distilled water throughout 3 days (Snakeskin Dialysis Tubing 3.5 KDa MWCO, Thermo Scientific), performing eight changes of water (4 °C). The resulting 7 wt% SF dissolution was recuperated and concentrated by means of dialysis against 30 wt% PEG (11 000 Da) for 24 h, in order to reach a concentration of 20–21 wt% of SF. This dissolution was used for the electrospinning experimentations.

2.3. Electrospinning and post-treatment of SF/TiO₂ mats

The electrospinning setup utilized comprised of an isolated cabin which contained an electrically-charged needle located through a focal plate, a syringe pump, and a circular collector (metallic), whose surface (∼375 cm²) was protected using aluminium foil. The electrospinning conditions were controlled to facilitate the stabilization of the Taylor cone. The voltage applied to the capillary needle was 19–20 kV and −1.5 kV to the metallic collector. The distance between the collector and the tip of the needle was 42 cm, and the injection rate of the SF dissolution was 1 mL h⁻¹.

The TiO₂ nanoparticles, contained in a methanolic suspension, were sprayed over the surface of the meshes during the electrospinning experiments by means of an atomizer as it is explained below.

SF mats with TiO₂ (SF/TiO₂) were produced from the electrospinning of 6 mL of 20 wt% SF dissolution. 30 mL of methanol containing 1500 mg of TiO₂ nanoparticles were sprayed during the electrospinning; 7 rounds of atomization were performed (4.3 mL per round) covering in a uniform way the whole surface of the collector. Before the first atomization and after the last one, 1.5 mL of SF dissolution were electrospun in order to produce a compact net avoiding the loss of the nanoparticles. These rounds of atomization were periodically distributed in order to guarantee the homogeneous presence of TiO₂ in the materials.

Negative controls of pure SF were produced without including nanoparticles. After production, all the electrospun mats were submitted to the annealing step performed by means of immersion in absolute methanol (30 min), in order to promote a transition of secondary structures of fibroin, increasing the β-sheet content. The mats were located among two portions of filter of paper, to ease drying and to avoid the mats from twisting. All the mats produced were gently washed with distilled water before the experiments.

2.4. X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy analysis (XDS) and surface area (SBET)

X-Ray Diffractometry (XRD), field emission scanning electron microscopy (FE-SEM), X-Ray photoelectron spectroscopy (XPS, K-ALPHA, Thermo Scientific) and energy dispersive X-ray spectroscopy analysis (EDS) were used to characterize the crystalline structure, morphology, samples surface and elemental analysis plus quantitative mapping of the photocatalysts, respectively, according to the method previously described by Garrido et al.⁴ The nitrogen adsorption isotherms were obtained at 77 K using a Quadrasorb SI-MP by Quantachrome. Outgassing was performed with a Masterprep Degasser (Quantachrome Corp.) at 120 °C for 12 h for SF and SF/TiO₂. Specific surface areas (SSA) were determined with the Brunauer–Emmett–Teller (BET) method using the N₂ adsorption/desorption isotherm data.

2.5. Attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR)

ATR-FTIR was employed with the purpose of confirming the change of SF to a non-soluble state (enriched in β-sheet structures), as well as to corroborate the presence of the nanoparticles in the materials produced, identifying the characteristic bands of TiO₂. Spectra were acquired on a Nicolet iS5 spectrometer, with an iD5 ATR accessory (Thermo Scientific, USA) using OMNIC software (Ver. 9.3.30), determining in absorbance mode with a resolution of 4 cm⁻¹, a spectral range of 4000–550 cm⁻¹, and 64 scans.

2.6. Evaluation of mechanical properties and fibre diameter of electrospun materials

A universal test frame machine (Qtest; MTS Systems, Eden Prairie, MN, USA) was used to carry out the tensile tests. The mechanical properties of the samples (10 mm × 30 mm) were proved setting a crosshead speed of 0.1 mm s⁻¹ and employing a load cell of 10 N. The width of each sample was measured with a digital micrometer (Mitutoyo Digimatic Micrometer 0–25 mm, resolution of 0.001 mm and a precision of ±2 μm). The elastic modulus (MPa), tensile strength (MPa) and strain at break (%) were calculated from the resultant stress–strain curves. Elastic modulus was calculated in the linear elastic part of the curves. Each test was accomplished at least three times per material. Additionally, SEM micrographs were used to measure the fibre diameters of the studied materials by means of image analysis (ImageJ software).

2.7. Photoreaction setup

Both trials (photocatalytic and photolytic) were carried out during August 2018, following the procedure described by Fenoll et al. with some modification.²⁴ A square fragment of SF/TiO₂ mats was introduced in each vessel containing 100 mL of water. Milli-RX water (pH 7.13, ORP 230 mV, resistivity > 5 MΩ cm (25 °C); conductivity 5.5 μS cm⁻¹, TOC < 30 μg L⁻¹; microorganisms < 10 cfu mL⁻¹) was spiked at 0.1 mg L⁻¹ of each pesticide with commercial formulations and exposed to solar radiation for 180 min (from 11 AM to 2 PM). The vessels were adequately separated to avoid shielding effect. Previously to the beginning of the trials the mixture was stirred for 30 min in the dark and a sample was withdrawn to measure the dark adsorption of pesticides onto each square fragment of SF and SF/TiO₂ mats. Fig. 1 shows a schematic drawing of the experimental system used. The values (all in W m⁻²) of VIS plus NIR, UVA and UVB at noon were 948.6 ± 52.4, 21.5 ± 2.3 and 1.7 ± 0.4, respectively.

Fig. 1. Schematic drawing of the experimental setup.

2.8. Analytical determinations

The method published by Fenoll et al.²⁵ was used to analyse pesticide residues in water samples using an HPLC system (Agilent Series 1200 Agilent Technologies, Santa Clara, CA, USA) and a G6410A triple quadrupole mass spectrometer equipped with an ESI interface operating in positive ion mode. Table 2 lists the analytical conditions of the studied pesticides. In addition, the method described by Fenoll et al.²⁴ was used to determine Ti by an Agilent 7900 ICP-MS with MicroMist glass concentric nebulizer, standard nickel cones, and Ultra High Matrix Introduction (UHMI) system. Finally, the method described by Fenoll et al.²⁴ was used to measure the dissolved organic carbon (DOC) content in water samples with a Multi N/C 3100 TOC Analyzer (Analytic Jena AG, Jena, Germany) equipped with an NDIR detector (950 °C).

Analytical conditions of the studied pesticides.

Compound	t R (min)	SRM1	Fragmentor1 (V)	Collision energy1 (V)	SRM2	Fragmentor2 (V)	Collision energy2 (V)
Boscalid	25.80	343 → 307	130	20	343 → 140	130	20
Pyraclostrobin	29.20	388 → 163	90	25	388 → 194	90	10
Hexythiazox	32.70	353 → 168	110	25	353 → 228	110	15
Trifloxystrobin	30.70	409 → 186	90	15	409 → 206	90	10

2.9. Statistical analysis

Curve fitting and statistical analyses was carried out using SigmaPlot version 13.0 statistical software (Systat, Software Inc., San Jose, CA). For the statistical analyses of mechanical properties and fibre diameter IBM SPSS 25 statistics software was used. Data were compared with ANOVA (p < 0.05) or Mann–Whitney (p < 0.05) tests depending if they accomplished normality and homoscedasticity requirements or not, respectively.

3. Results and discussion

3.1. Field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD)

XRD patterns of SF, SF/TiO₂ and TiO₂ P25 samples are shown in Fig. 2. No representative peaks were observed for SF. By contrast, SF/TiO₂ sample show intensive (Int/100 > 10) diffraction peaks at ca. 2θ of 25.3, 37.8, 48.0, 53.9, 55.1, 62.8, which corresponds to the 101, 004, 200, 105, 211, and 204 planes, all of them characteristics of anatase (ICDD PDF2 database Set-file 21-1272). For rutile phase, the higher peak (Int/100 > 10) corresponds to 2θ of 27.4 (110 plane), typical peak of rutile (ICDD PDF2 database Set-file 21-1276). Although it can be considered that the surface coverage is complete, a decrease in the overall intensity for SF/TiO₂ was observed in comparison to TiO₂ P25, which implies lower crystallinity.

Fig. 2. XRD patterns of SF/TiO₂, SF and TiO₂.

The SEM images of SF and SF/TiO₂ are shown in Fig. 3. It shows a non-uniform TiO₂ layer around the SF. In addition, the SEM image of the SF/TiO₂ shows a cluster of aggregates of TiO₂ deposited on the SF with an average size of approximately 20–30 nm. Again this is consistent with the adsorption of P25 aggregates and with the data for P-25 TiO₂ provided by Degussa Co. and other researchers.^4,26,27 The elemental mapping of SF/TiO₂ showed that the surface coverage of the SF with TiO₂ was practically complete (Fig. 4).

Fig. 3. SEM images of SF (A and C) and SF/TiO₂ (B and D).

Fig. 4. Back-scattered electron (BSE) image (A), elemental mapping (B) and EDX data (C) of a SF/TiO₂ zone.

The surface chemical composition of SF/TiO₂ and SF samples were further analyzed by XPS (Fig. 5 and 6). The survey spectrum of SF/TiO₂ and SF (Fig. 5A and 6A) shows the presence of Ti, O, N and C elements and O, N, C elements, respectively. The binding energies for the Ti 2p_1/2 and Ti 2p_3/2 were 464.3 and 458.4 eV, respectively, whereas in pure TiO₂ P25, these binding energies were 463.9 and 458.2 eV, respectively⁴ (Fig. 5B). The O 1s spectra are also shown in Fig. 5C and 6C. The O 1s peak of SF/TiO₂ could be resolved into three peaks by the XPS peak fitting program. The presence of different oxygen species could explain the asymmetric nature of O 1s peaks.²⁸ The XPS analysis result is in accordance with the result of the XRD pattern. In addition, similar C 1s spectra are obtained for SF/TiO₂ and SF (Fig. 5D and 6D). Finally, the binding energy for the N 1s was 339.8 eV (Fig. 6B).

Fig. 5. XPS spectra of SF/TiO₂ sample: (A) survey spectrum, (B–D) high-resolution binding energy spectra of Ti 2p, O 1s and C 1s, respectively.

Fig. 6. XPS spectra of SF sample: (A) survey spectrum, (B–D) high-resolution binding energy spectra of N 1s, O 1s and C 1s, respectively.

Gas physisorption isotherms of nitrogen or argon adsorbates are the basis to measure specific surface area (SSA) of porous material and pore size distribution.²⁹ In the case of SF (2 m² g⁻¹) almost no N₂ adsorption is observed, suggesting that the pore structures are very poor. Fig. 7A and B shows the N₂ adsorption/desorption isotherms and BET surface area plot of SF/TiO₂.The creation of pores and the increase of SSA in SF/TiO₂ is supported by the N₂ physisorption isotherms which show that SSA is increased to 31 m² g⁻¹, which is close to the SSA obtained for TiO₂ P25 (55 m² g⁻¹).²⁶

Fig. 7. (A) N₂ adsorption–desorption isotherms and (B) BET surface area plot of SF/TiO₂.

3.2. Attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR)

The results of ATR-FTIR analysis confirmed that the TiO₂ nanoparticles were incorporated effectively in the materials. Along the spectra obtained from the SF/TiO₂ meshes (Fig. 8, green line) are observed both, the characteristic bands of SF (Fig. 8, blue spectrum) and the corresponding to TiO₂ (Fig. 8, red line).

Fig. 8. Infrared spectra of pure fibroin mats (SF), mats containing TiO₂ nanoparticles (SF–TiO₂) and pure TiO₂ nanoparticles (TiO₂).

SF and SF/TiO₂ mats exhibited the peaks corresponding to amide I (1622 cm⁻¹), amide II (1515 cm⁻¹), amide III (1230 cm⁻¹) and amide IV (1064 cm⁻¹), highlighted with blue arrows in Fig. 7, these are widely described for fibroin biomaterials in β-sheet conformation.^30,31 The peak at 1660 cm⁻¹ did not appear, this fact verified that the conformation of the fibroin transformed from random coil to β-sheet;³² which implicates a transition to a non-soluble state in water. Similarly, as mentioned above, the spectra of SF/TiO₂ meshes showed a broad band at 700 cm⁻¹ caused by the presence of TiO₂ in their composition (highlighted with a red arrow). This band, present also in the spectrum of pure TiO₂, is assigned to the vibrations caused due to (Ti–O–Ti) stretch.^21,33

3.3. Mechanical properties and fibre diameters

The characterization of the mechanical properties and the diameter of the fibres of the mats produced was carried out in order to evaluate if the incorporation of the TiO₂ nanoparticles affected the properties of the fibroin materials. In the case of the diameter of the fibres, the homogeneous incorporation of a layer of TiO₂ gave rise to a statistically significant increase in their average values (Mann–Whitney, p < 0.05). Pure fibroin meshes presented an average value of fibre diameter of around 2.9 microns and those that included TiO₂ around 3.9 microns.

On the other hand, the values obtained for the mechanical properties were not significantly altered by the incorporation of TiO₂. These are described in Table 3 and are in the range of the ones stated in other works studying electrospun SF materials.^16,34 The tensile strength ranged between 0.3–0.4 MPa, the strain at break was estimated around 1.8–2.3% and the elastic modulus presented values between 23–24 MPa.

Average values of fibre diameter and mechanical properties studied in SF and SF/TiO₂ electrospun materials.

Mats	Fibre diameter (nm)	Tensile strength (MPa)	Elastic modulus (MPa)	Strain at break (%)
SF	2948 ± 748	0.409 ± 0.119	24 238 ± 8126	2278 ± 0.256
SF/TiO2	3858 ± 1167a	0.331 ± 0.088	22 770 ± 5056	1848 ± 0.271

^aStatistically different values compared to negative control (Mann–Whitney, p < 0.05).

3.4. Photocatalytic activity and kinetics

The effect of TiO₂ loading on the disappearance kinetics of the different pesticides was evaluated in order to know maximum degradation efficiency. The experiment was carried out using three square fragments of SF/TiO₂ mats (2.25, 6.25 and 12.25 cm²) with different TiO₂ amounts (9, 25 and 50 mg), respectively. Dark adsorption was minimal and similar for the studied insecticides on both square fragments of SF and SF/TiO₂ mats, with different size, after 30 min of incubation. The degradation rate significantly increases by adding TiO₂. However, no significant differences (p < 0.05) were observed when TiO₂ loading was increased from 25 to 50 mg for most compounds (except boscalid) (Fig. 9). Therefore, SF mats with 25 mg of TiO₂ were selected. The normalized illumination time (t_{30 W}) was used instead of exposure time (t) to compare data from several days' and other photocatalytic experiments according to the following equation (eqn (1)).

t_{30 W, n} = t_{30 W, n−1} + Δt_n(UV/30)(V_i/V_T) Δt_n = t_n − t_n−1 1

Fig. 9. Optimization of TiO₂ load for photocatalytic degradation of studied pesticides (0.1 mg L⁻¹). Error bars denote standard deviation (n = 3).

This equation has been used by several authors working on this topic,^35,36 where each parameter is described.

In this case, the photodegradation of boscalid, pyraclostrobin, hexythiazox and trifloxystrobin using SF/TiO₂ was evaluated (Fig. 10).

Fig. 10. Degradation kinetics in water of the studied pesticides (0.1 mg L⁻¹) by photolysis (with and without SF) and heterogeneous photocatalysis (SF/TiO₂ and TiO₂ nanopowder) during the photoperiod. Approximately 250 mg L⁻¹ of TiO₂ in all cases. Error bars denote standard deviation (n = 3).

The residual levels of boscalid, pyraclostrobin, hexythiazox and trifloxystrobin after 180 min (t_{30 W} = 146 min) of light exposure were 44.4, 17.3, 12.0 and 14.8 μg L⁻¹, respectively. In contrast, the photolytic decomposition of the pesticides with and without SF mats occurred at a lower rate than that observed in the photocatalytic process, and only a decrease from 58–98% of the initial concentration was achieved for hexythiazox and boscalid, respectively, after 180 min of lighting (t_{30 W} = 146 min).

The dissolved organic carbon (DOC) content was measured to investigate the mineralization degree for the studied pesticides through the experiment. The initial and final content of DOC were 4.3 mg L⁻¹ and 1.3 mg L⁻¹, respectively. This mineralization degree could be due to the formation of non-degradable intermediates during the solar treatment and to the presence of the coadjuvants in the commercial formulations.

The kinetic parameters of boscalid, pyraclostrobin, hexythiazox and trifloxystrobin for SF/TiO₂ and TiO₂ nanopowder are shown in Table 4. The photooxidation of the studied pesticides follows pseudo first-order behaviour consistent with the Langmuir–Hinshelwood model (C_P = C_P0 e^−k_apt, where C_P is the concentration of the pesticide and k_ap is the apparent first-order rate constant), with R² values, using exposure time (t), in all cases higher than 0.938 for the four pesticides.

Kinetic parameters for photocatalysis (SF/TiO₂ and TiO₂) of pesticides in water under natural sunlight.

Pesticide	SF/TiO2			TiO2
	R 2	k × 102 (min−1)	t ½ a (min)	R 2	k × 102 (min−1)	t ½ a (min)
Boscalid	0.859	0.867	79.7	0.908	1.904	36.5
Pyraclostrobin	0.897	1.836	36.7	0.827	3.217	21.5
Hexythiazox	0.731	2.506	27.6	0.759	5.287	13.1
Trifloxystrobin	0.841	2.398	28.9	0.782	4.615	15.0

^aReferred to t_{30 W} (calculated according eqn (1)).

The rate constants for the photocatalytic oxidation of pesticides by SF/TiO₂ and TiO₂ nanopowder, using normalized illumination time (t_{30 W}), varied from 0.009–0.025 and 0.019–0.053 min⁻¹, respectively. The greater S_BET of the TiO₂ nanopowder and the different impact and level of illumination between SF/TiO₂ and TiO₂ nanopowder could explain these differences in rate constants.²⁸ The smaller particle size causes larger S_BET. Therefore, much more space is unoccupied for the adsorption of pesticides, with the consequent improvement of the effectiveness of photocatalytic process.

The analysis of Ti into solution after the solar irradiation was carried out by ICP-MS. The presence of Ti in water during the irradiation time was found to be negligible according to previous papers.^4,26,28

Reusability study of SF/TiO₂ was done to ensure its applicability as recycled catalyst. The photooxidation of boscalid, pyraclostrobin, hexythiazox and trifloxystrobin was performed for 720 min with sampling every 180 min (4 cycles). The decrease of rate constants for photocatalytic degradation was lower than 12% in the most unfavourable case (n = 3). This fact was probably due to the accumulation of organic intermediates and coadjuvants in the cavities and on the surface of the catalyst affecting the adsorption of pesticides, and reducing the activity of SF/TiO₂.

4. Conclusions

Electrospun SF/TiO₂ mats were synthesized and characterized. These materials were applied for the photocatalytic degradation of four pesticides (boscalid, hexythiazox, pyraclostrobin and trifloxystrobin) in water under sunlight. The photooxidation of these pesticides followed first order kinetics. A reusability study of SF/TiO₂ showed that the decrease of rate constants for photocatalytic degradation was lower than 12%. From the results of this work, it can be concluded that solar photocatalytic degradation using electrospun SF/TiO₂ mats can be used to remove pesticides from water in different applications. Future research should address the efficacy of the electrospun SF/TiO₂ mats with respect to other pollutants and kinds of waters.

Conflicts of interest

There are no conflicts to declare.