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. 2022 Apr 12;25(5):104241. doi: 10.1016/j.isci.2022.104241

Figure 6.

Figure 6

High dietary tryptophan promotes mTOR activation in CD4+ T cells

(A–F) MFI of p4EBP1, pS6, and pAKTSer473 in Tem (A–C) and Treg (D–F) cells from TC mice fed low or high tryptophan for 1 month, N = 4.

(G) Representative Western blot analysis of TC Teff and Treg cells for p4EBP1 and β-actin. For Teff samples, high tryptophan group is on the left. For Treg samples, high tryptophan group is on the right.

(H–I) Quantification of Western blot results for p4EBP1 relative to β-actin, N = 5.

(J) Representative immunofluorescence staining for CD4, mTOR, and IgD in spleen sections from TC mice fed high or low tryptophan for 2 months. Arrow heads indicate mTOR+ CD4+ T cells. Graphs show individual mice with mean ± SEM t tests, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.