Microbiology Correction for “Herpesvirus-mediated stabilization of ICP0 expression neutralizes restriction by TRIM23,” by Xing Liu, Dhiraj Acharya, Eric Krawczyk, Chase Kangas, Michaela U. Gack, and Bin He, which published December 13, 2021; 10.1073/pnas.2113060118 (Proc. Natl. Acad. Sci. U.S.A. 118, e2113060118).
The authors note that Fig. 6E appeared incorrectly. The corrected figure and its legend appear below. The online version has been corrected.
Fig. 6.
TRIM23 inhibits replication of HSV deficient in ICP0. (A) Viral mRNA expression analysis. TRIM23+/+ and TRIM23−/− MEFs were infected with the indicated viruses (HSV-1, R7910, or R7911) at an MOI of 5 for 12 h and harvested for qRT-PCR analysis of ICP4, ICP27, gC, and gD. (B) Viral protein production in TRIM23+/+ and TRIM23−/− MEFs that were mock infected or infected as in A at 12 h post infection. Cells were harvested for Western blot analysis with antibodies against ICP4, ICP0, ICP27, gC, gD, and β-actin. (C) IF analysis of gC in TRIM23+/+ and TRIM23−/− MEFs that were mock infected or infected with viruses as in A and then stained with gC antibody (green) and DAPI (blue). (D) Quantification of gC. IF images in C were quantified using NIH ImageJ software (set the HSV-1 in TRIM23+/+ group as 1.0). (E) Viral replication in TRIM23+/+ and TRIM23−/− MEFs that were infected with the indicated viruses (MOI = 0.01). At 48 h postinfection, viral yields were titrated by plaque assay on U2OS cells. (F) Effect of TRIM23 on viral replication. TRIM23 in TRIM23−/− cells were transiently transfected with Flag-Vec or Flag-TRIM23 for 24 h. Cells were then infected with HSV-1, R7910, or R7911 (MOI = 0.01). Viral yields were determined by plaque assay on U2OS cells at 48 h postinfection. One-way ANOVA was used for A, D, E, and F, **P < 0.01. Data are expressed as means ± SD (n = 3) in A, E, and F and quantified from at least an average 10 images per group in D. The data shown in A, B, E, and F are representative of results from three independent experiments. The images are representative of results from at least 10 images for C.