Abstract
Correction for ‘Base recognition by l-nucleotides in heterochiral DNA’ by Shuji Ogawa et al., RSC Adv., 2012, 2, 2274–2275.
The authors regret that some of the data in the original article were presented incorrectly. Some of the oligonucleotide sequences in the Graphical Abstract, Fig. 2 and Table 1 were originally presented in reverse sequence. The corrected versions of the Graphical Abstract, Fig. 2 and Table 1 are presented below.
Fig. 2. Effects of base pair mismatch of d- (a–d) and l-nucleotide (e–h) on duplex stability. Samples contained 6 mM duplex in 10 mM MgCl2, 100 mM NaCl, and 70 mM MOPS (pH 7.1). Yellow bars denote Tm values of fully matched duplexes, and blue bars denote Tm values of mismatched duplexes.
UV-melting points of homo- and heterochiral duplexesa.
| Duplex | Template strand | Complementary strand | T m (°C) | ΔTmb (°C) |
|---|---|---|---|---|
| Homochiral strand | ||||
| 1 | d(AAATCTGCG) | d(CGCAGATTT) | 42.1 | — |
| Heterochiral strand | ||||
| 2 | d(AAlATCTGCG) | d(CGCAGATTT) | 33.6 | −8.5 |
| 3 | d(AAATCTlGCG) | d(CGCAGATTT) | 32.6 | −9.5 |
| 4 | d(AAATlCTGCG) | d(CGCAGATTT) | 38.2 | −3.9 |
| 5 | d(AAAlTCTGCG) | d(CGCAGATTT) | 33.9 | −8.2 |
a
Samples contained 6 μM duplex in 10 mM MgCl2, 100 mM NaCl, and 70 mM MOPS (pH 7.1).
b
Melting temperature difference from the homochiral duplex.
The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.