Video 12. Epithelial retreat and respreading.

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Movie from which Figure 4E was made. Animal cap epithelium, recombined with ventral deep MZ. Under cover glass the entire time. Note that deep IMZ cells spread animally over the deep NIMZ tissue between frames 1 and 30 (first 20% of the movie), most obviously at the lateral edges; in response the more vegetal superficial cells over the IMZ initially contract; during the next 20% of the movie, the superficial cells move animally and begin to respread over the NIMZ. Stage 10.25–15 (G + 1 h to G + 8.5 h). 3 min/frame. Scale bar = 100 μm.