| Antibody |
pMLC 2 (Ser19)(Rabbit monoclonal) |
Cell Signaling |
Cat. #3,671RRID:AB_330248
|
(1:100) |
| Antibody |
6B6(recognizes C-cadherin)(mouse monoclonal) |
Developmental Studies Hybridoma Bank |
AB_528113RRID:AB_528113
|
(1:300) |
| Other |
phalloidin-Alexa 555 |
(Acti-stain, Cytoskelton.com) |
8,953 |
Derivatized fluorescent dextran (1:100 at 4 C, 12–36 hours) |
| Sequence-based reagent |
MHC IIB morpholino |
Skoglund et al., 2008
|
|
Morpholino5’CTTCCTGCCCTGGTCTCTGTGACAT3’ |
| Sequence-based reagent |
Xdd1 |
Sokol, 1996
|
|
RNA |
| Sequence-based reagent |
dnWnt11 |
Tada and Smith, 2000
|
|
RNA |
| Software, algorithm |
NIH Image 1.6 |
Wayne Rasband, National Institutes of Health; available at http://rsb.info.nih.gov/nih-image/
|
RRID:SCR_003073
|
|
| Software, algorithm |
Object Image |
Norbert Vischer, University of Amsterdam; available at https://sils.fnwi.uva.nl/bcb/Object-Image/object-image.html
|
RRID:SCR_015720
|
|
| Software, algorithm |
Image J |
http://rsb.info.nih.gov/ij/
|
RRID:SCR_003070
|
|
| Software, algorithm |
Metamorph |
https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy#gref
|
RRID:SCR_002368
|
|
| Software, algorithm |
SquisherJoy |
(CellScale, Waterloo, Canada) |
Software for MicroSquisher; RRID:SCR_022034
|
|
| Software, algorithm |
Axisymmetric Drop Shape Analysis |
del Río and Neumann, 1997
|
ADSA in MatlabRRID:SCR_001622 for Matlab |
|
| Software, algorithm |
curvature outline detection |
https://www.mathworks.com/products/matlab.html
|
(Canny) in MATLABRRID:SCR_001622 for Matlab |
|
| Software, algorithm |
Surface tension determination in SquisherJoy |
Based on Brodland et al., 2009; Mgharbel et al., 2009
|
|
|
