Skip to main content
. 2022 Apr 14;11:e70664. doi: 10.7554/eLife.70664

Figure 9. The Clstn3 KO in cerebellar cortex increases the amplitude and frequency of parallel-fiber mEPSCs.

Figure 9.

(A–C) The cerebellar Clstn3 KO increases the amplitude and frequency of mEPSCs in Purkinje cells (A, representative traces; B, left, cumulative probability plot of the mEPSC amplitude right, average amplitude; C, cumulative probability plot of the mEPSC inter-event interval [inset, average frequency]). (D) The cerebellar Clstn3 KO has no effect on mEPSC kinetics (left, mEPSC rise times; right, mEPSC decay times). (E) Expanded traces of averaged mEPSCs to illustrate the kinetic similarity of control and Clstn3 KO events with a change in amplitude. (F & G) mEPSCs with slow rise times ( > 1ms) and that are likely primarily derived from parallel-fiber synapses exhibit the same phenotype as the total mEPSCs (same as B and C, but for mEPSCs with slow rise times). (H) The cerebellar Clstn3 KO has no effect on the kinetics of mEPSCs with slow rise times (left, mEPSC rise times; right, mEPSC decay times). All numerical data are means ± SEM. Statistical significance with two groups was assessed by unpaired t-test (*p < 0.05, **p < 0.01), with the number of cells/mice analyzed indicated in the first bar graphs for each experiment. Cumulative analysis was done with Kolmogorov-Smirnov test (***p < 0.001).