Fig. 2.

Interaction of MAR mutants with Nup60 in the Y2H assay. Panels show Y2H assays of wild-type and mutant MARs interacting with either full-length Nup60 or Nup60(188–388). A pGAL1-HIS3 strain transformed with bait and prey plasmids was spotted onto -Trp -Leu (left panels) and -Trp -Leu -His + 5 mM 3-AT plates (right panels). The MAR alleles used as bait are indicated on the left-hand side; the fragments of Nup60 used as prey are indicated on the top. Bait controls were included on all the plates but are only shown for the top panels. Although at least 3 independent transformants were tested for each bait/prey combination, only one sample is shown.