Fig. 7.

Lipid analysis of DOG-IM4 LNPs by UHPLC-CAD-MS at T0 and after 18 weeks of storage at different temperatures. The LNPs used in the stability study described inFig. 7were analyzed for lipid integrity by UHPLC-CAD-MS. PanelA: CAD chromatogram at T0; PanelB: CAD chromatograms after 18 weeks at 4°C (in red), 25°C (in blue) or 37°C (in black). The slight shift in retention times observed between T0 and T18 weeks chromatograms is due to the replacement of the HPLC column by a new one. PanelC: Total Ion Current and mass spectra after 18 weeks at 4°C (in red), 25°C (in blue) or 37°C (in black). The mass spectra correspond to the retention time of DOG-IM4 (13.1 min; indicated by the black star).