KEY RESOURCE TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
rabbit anti-RCFP	Takara	632475
Alexa Fluor 568 goat anti-rabbit IgG (H+L)	Thermo Fisher Scientific	A-11036
Bacterial and virus strains
AAV2-hSyn-hChR2(H134R)-mCherry	Karl Deisseroth Lab	UNC Vector Core N/A
pENN-AAV-CAG-tdTomato-WPRE-SV40	UPenn Vector Core	Addgene_105554-AAV1
pENN-AAV1-CamKII(0.4)-CRE-SV40	UPenn Vector Core	Addgene_105558-AAV1
AAV1-hsyn-DIO-TVA66T-dTom-CVS-N2C (g)	Allen Brain Institute	N/A
EnvA dG CVS-N2C Histone-eGFP	Allen Brain Institute	N/A
Chemicals, peptides, and recombinant proteins
Tetrodotoxin	Abcam	ab120055; CAS: 4368-28-9
Tetrodotoxin	Tocris	1078; CAS: 4368-28-9
D-APV	Tocris	0106; CAS: 79055-68-8
4-AP	Sigma-Aldrich	CAS:504-24-5
(+)-MK 801	Tocris	0924; CAS: 77086-22-7
DNQX disodium salt	Tocris	2312; CAS: 1312992-24-7
Picrotoxin	Tocris	1128; CAS: 124-87-8
MNI-caged-L-glutamate	Tocris	1490; CAS: 295325-62-1
OGB-6F	Invitrogen	O23990
CPP	Sigma-Aldrich	C104; CAS: 126453-07-4
Alexa 488	Invitrogen	A10436
Alexa 594	Invitrogen	A10438
DAPI	Thermo Fisher Scientific	62248
Experimental models: Organisms/strains
C57/bl6 mice	Jackson Labs	RRID: IMSR_JAX:000664
Software and algorithms
MATLAB (R2018a)	MathWorks	https://www.mathworks.com
ImageJ	National Institutes of Health	https://imagej.nih.gov/ij/index.html
Prism	Graphpad	RRID:SCR_002798
Other
Dagan BVC-700A	Dagan Corporation	N/A
Electro-optical modulator	Conoptics	M350–50
Leica VT1200 S Fully automated vibrating blade microtome	Leica	VT1200 S
MaiTai DeepSee	Spectra-Physics	MAI TAI HP DS
Photosensor module	Hamamatsu	H7422A-40
Ultima In Vitro Multiphoton Microscope System	Bruker	N/A
Collimated LED for Olympus BX	ThorLabs	M625L4-C1
473 nm laser	OptoEngine LLC	MSL-FN-473/1~100mW
TCS SP8 upright confocal microscope	Leica	DM6000