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. 2021 Dec 9;220(2):iyab212. doi: 10.1093/genetics/iyab212

Figure 3.

Figure 3

Hotspot-binding/activating proteins are rate-limiting for promoting recombination at their own DNA binding sites. Recombination was measured in crosses that were homozygous wild-type, heterozygous wild-type/null mutant, and homozygous null mutant for hotspot-activating proteins. The heterozygous crosses were in two configurations. In the first configuration (+/Δ), the parent with the hotspot allele was wild-type for the binding protein (e.g., genotype ade6-M26 atf1+) and the parent with the test-cross allele was null mutant for the binding protein (e.g., ade6-M210 atf1Δ). In the second configuration (Δ/+), the parent with the hotspot allele was null mutant for the binding protein (e.g., ade6-M26 atf1Δ) and the parent with the test-cross allele expressed the binding protein (e.g., ade6-M210 atf1). Rates of recombination are plotted relative to those in homozygous wild-type and are grouped by class of hotspot: (A) M26 DNA site; (B) CCAAT DNA site; (C) Oligo-C DNA site. Data are mean from three or more independent biological replicates; differences with P ≤ 0.05 are indicated for mutant vs wild-type (*).