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. 2022 Apr 27;11(9):1468. doi: 10.3390/cells11091468

Figure 3.

Figure 3

Reciprocal regulation between ANRIL and p15. Mice p15 gene was cloned upstream of the luciferase reporter. Mice ANRIL plasmid or siRNA were co-transfected with luciferase reporter into mouse TM cells. (A) The luciferase activity was greatly increased in siANRIL cells compared to scramble. In oANRIL cells, the luciferase activity was suppressed. (B) Depletion of ANRIL increased the mRNA level of p15 in TM cells, and overexpression of ANRIL inhibited the expression of p15, as measured by real-time PCR. (C) Depletion of ANRIL increased the p15 protein level in TM cells. Notes: n = 6 per group; oANRIL = ANRIL overexpression; siANRIL = ANRIL knock down; DEX = dexamethasone; * p < 0.05 compared with vector; ** p < 0.01 compared with vector; ## p < 0.01 compared with scramble RNA.