Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 12;159:21. doi: 10.1186/s41065-022-00235-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — Overexpression of IL-6 reversed the effect of USP14 shRNA on ferroptosis of LPS-treated GMECs. LPS-treated GMECs were transfected with USP14 shRNA alone or together with CMV-IL-6. A-C The expression of USP14, IL-6, and GPX4 protein was detected by Western blotting and the grayscale was analyzed by using ImageJ software. D Cell viability was detected by CCK-8 assay. E The secretion of TNF-α was detected by ELISA. F The content of ROS was analyzed with DCFDA-cell ROS detection kit. G MDA content was detected by MDA assay kit. H The GSH levels were detected by GSH assay kit. I The concentration of Fe²⁺ was measured by using an iron assay kit