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. 2022 May 12;159:21. doi: 10.1186/s41065-022-00235-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Inhibition of NRF2 signaling pathway reversed the effect of IL-6 shRNA on ferroptosis of LPS-treated GMECs. LPS-treated GMECs were transfected with IL-6 shRNA alone or together treated with the NRF2 signaling protein inhibitor Brusatol. A-E The expression of NRF2 and its downstream pathway proteins were detected by Western blotting and the grayscale was analyzed by using ImageJ software. F Cell viability was detected by CCK-8 assay. G The secretion of TNF-α was detected by ELISA. H The content of ROS was analyzed with DCFDA-cell ROS detection kit. I MDA content was detected by MDA assay kit. J The GSH levels were detected by GSH assay kit. K The concentration of Fe²⁺ was measured by using an iron assay kit. L, M The expression level of GPX4 was detected by Western blotting and the grayscale was analyzed by using ImageJ software. N Represented images of transmission electron microscopy