Identification of a novel CNV at the EYA4 gene in a Chinese family with autosomal dominant nonsyndromic hearing loss

Abstract

Background

Hereditary hearing loss is a heterogeneous class of disorders that exhibits various patterns of inheritance and involves many genes. Variants in the EYA4 gene in DFNA10 are known to lead to postlingual, progressive, autosomal dominant nonsyndromic hereditary hearing loss.

Patients and methods

We collected a four-generation Chinese family with autosomal-dominant nonsyndromic hearing loss (ADNSHL). We applied targeted next-generation sequencing (TNGS) in three patients of this pedigree and whole-genome sequencing (WGS) in the proband. The intrafamilial cosegregation of the variant and the deafness phenotype were confirmed by PCR, gap-PCR and Sanger sequencing.

Results

A novel CNV deletion at 6q23 in exons 8–11 of the EYA4 gene with a 10 bp insertion was identified by TNGS and WGS and segregated with the ADNSHL phenotypes.

Conclusions

Our results expanded the variant spectrum and genotype‒phenotype correlation of the EYA4 gene and autosomal dominant nonsyndromic hereditary hearing loss in Chinese Han individuals. WGS is an accurate and effective method for verifying the genomic features of CNVs.

Supplementary Information

The online version contains supplementary material available at 10.1186/s12920-022-01269-x.

Introduction

Hearing loss is the most common sensory deficit in modern society and affects approximately 466 million people worldwide, 34 million of whom are children [1, 2]. Hereditary hearing loss patterns vary among autosomal dominant, autosomal recessive, X-linked, and mitochondrial patterns of inheritance. Unlike syndromic hearing losses, non-syndromic hearing loss are not associated with other clinical abnormalities [3]. In 75–80% of cases, NSHL is inherited autosomally recessively, while 20% are inherited autosomally dominantly. X-linked (2–5%) or mitochondrial patterns of inheritance (1%) are rare in NSHL [3, 4]. The main characteristics of autosomal dominant hearing loss are high genetic and clinical heterogeneity and a delayed onset (postlingual), which are easily overlooked during hearing screening of newborns. At present, 51 different genes and 67 different loci have been linked to autosomal-dominant NSHL [5].

The EYA4 gene, which is located on chromosome 6q22.3-q23.2, was first identified as a causal gene of DFNA10 in a large American family in 1996 [6]. The EYA4 gene encodes eye absent 4 protein and is considered necessary for the proper development of multiple human organs, including the eye, inner ear, and heart. The EYA4 protein comprises two functional domains that contain a 271-amino-acid highly conserved C-terminal EYA domain (eyaHR) and an N-terminal variable region with a proline-serine-threonine (PST)-rich transactivation domain (eyaVR) [7]. By mediating interactions with the sine oculis family of proteins (Six1–Six6), mammalian EYA proteins function as transcriptional coactivators [8].

Researchers from different countries have found novel variants of the EYA4 gene and deletions of the EYA4 allele in different families with hearing loss based on sequencing analysis [9–14]. Late-onset, progressive, sensorineural hearing loss and age of onset from 6 to 50 years are the common characteristics among the tested families. Mid-frequency hearing is affected first, and all frequencies gradually become affected with increasing age. The degree of hearing loss also ranges from mild to moderate or severe with spontaneous evolution [15].

In recent years, next-generation sequencing (NGS) technology, including both targeted and whole-genome sequencing (WGS), has been considered an efficient and swift method to detect potential variants [16, 17]. This method provides a guiding role in the diagnosis and treatment of hereditary hearing loss [18]. Copy number variations (CNVs) are genomic variants within species that reflect differences in copy numbers, including deletions, duplications, and amplifications of DNA sequences. By using cytogenomics techniques such as comparative genomic hybridization (CGH), SNP arrays, WES and WGS, many novel CNVs associated with NSHL phenotypes have been identified [19–23]. With the development of diagnostic WGS, the accessibility, robustness and accuracy of CNVs throughout the genome have dramatically improved. Compared to genomic microarrays, the investigation of genomic features such as copy number, content and positional information has become more precise through the WGS method and algorithm [24, 25].

In the present study, we present a four-generation Chinese pedigree with autosomal dominant nonsyndromic hearing loss. A novel CNV deletion at 6q23 was identified in the affected individuals by targeted next-generation sequencing (TNGS) and WGS, and this information sheds new light on the pathogenic mechanism of EYA4 variants.

Patients, materials and methods

Family and clinical evaluation.

A Chinese family (FY-140) classified as of Han origin presented with late-onset, progressive hearing loss. Approval for the study was obtained from the Ethics Committee of the Eye & ENT Hospital, Fudan University for Human Studies. Written informed consent was obtained from the participants or the parents of minors. The assessment of all the individuals was based on audiological methods, including pure-tone audiometry, acoustic impedance, auditory brainstem response (ABR), distortion product of otoacoustic emission (DPOAE) and otological examination. Clinical information, such as age of onset, degree of hearing loss, progression of hearing loss, noise exposure and history of using aminoglycosides, was collected from family members if available. Information on deceased family members was obtained from relatives. The proband (II-2) was subjected to a high-resolution CT scan of the temporal bone. All the individuals accepted electrocardiography for the reason that some references indicated that EYA4 gene variants may cause heart disease in patients.

Targeted genomic capturing and next-generation sequencing

Genomic DNA was isolated using the TIANamp Blood DNA Midi Kit (TIANGEN Biotech, Beijing China) and fragmented to 150 bp using an ultrasonoscope (Covaris S220, Massachusetts, USA). End repair, adenylation and adapter ligation were performed for library preparation using a standard library construction kit (MyGenostics Inc., Beijing, China). Targeted DNA fragments were captured by a sequence capture array (MyGenostics Inc., Beijing, China). High-throughput sequencing and processing and bioinformatic data analysis were performed using the DNBSEQ-T7 sequencing platform (MGI Tech Co, Shenzhen China). The raw sequence reads were filtered using the BWA MultiVision software package and then aligned to GRCh38/hg38 (University of California Santa Cruz version). SNPs and indels were identified using the GATK Indel Genotyper and ANNOVA software. A CNV analysis was performed using the log2 ratio of the read depth on each exon.

Whole-genome sequencing

The genomic DNA samples were fragmented by sonication to a size of 300–500 bp. Sequence analysis was performed using the TruSeq Nano DNAHT Sample Prep Kit (Illumina Inc., Massachusetts, USA) following the manufacturer’s instructions. The total effective data yield of the sample was approximately 430 million reads, and the data showed a coverage of > 99.62% at 20X. After the raw sequence reads were mapped to the human genome reference sequence (USSC) (GRCh38/hg38), SpeedSeq software was used. SNPs, indels, CNVs and SVs were captured using GATK HaplotypeCaller, ANNOVAR and SpeedSeq software.

PCR, gap-PCR and Sanger sequencing

PCR, gap-PCR and Sanger sequencing were performed to analyze the cosegregation of variants with NSHL in this family. All primers were designed with Primer3 software (Applied Biosystems). Gap-PCR was designed to detect certain CNVs of the EYA4 gene. The following seven PCR primers were used for analysis of the suspected variants: EYA4 gene (Forward1, 5′-ATGAAGCCAAACACATATATTTCAA-3′; Forward2, 5′-TAGTGGCTACAGCCCCAGATCA-3′; and Reverse, 5′-AAACATTTTGGATGACGTTCCAT-3′) and CDH23 gene (Forward1, 5′-CACCCAGGTGGTGATCCAAGT-3′; Reverse1, 5′-GGAGCAGGAGAGTAGCTCTGGTTG-3′; Forward2, 5′'-CAGTACCAGCTGCTGACAGTGC-3′; and Reverse2, AGCAGGGCATATGTGGGTCATCT-3′). The cycling program was as follows: 95 °C for 2 min; 11 cycles of 94 °C for 20 s, 64–0.5 °C per cycle for 40 s, and 72 °C for 1 min; 24 cycles of 94 °C for 20 s, 58 °C for 30 s, and 72 °C for 1 min; 72 °C for 2 min; and 4 °C for the rest of the time. The standard protocols for Sanger sequencing were performed using an ABI 3730XL Dx Genetic AnalyserAnalyzer (Applied Biosystems) and PolyPhred software to confirm the detected variants in patients (II-2, II-3, II-6 and III-1) and healthy family members (I-2, III-2, III-3, III-5, IV-1 and IV-2).

Results

Clinical evaluation

The pedigree of the family includes 15 members (eight men and seven women) over four generations (Fig. 1A). Five individuals were diagnosed with NSHL based on their medical history and audiological function examination results. The self-reported age of onset of hearing impairment ranged from 26 to 43 years. Assessments of the four affected living members showed mild to severe bilaterally symmetric NSHL across all frequencies, and the disease affected both sexes (Table 1). In addition to hearing loss, the patients had no other clinical symptoms or signs. No patients complained of vestibular symptoms. The temporal bone scans and cardiac examinations of the proband yielded normal results. The initial hearing loss showed an audiogram pattern called a “cookie bite”, which was usually mild and only affected mid-frequencies. Progressive hearing loss expanded to other frequencies at later stages (Fig. 1B). Other audiometric tests' results in affected individuals including ABR, DPOAE, acoustic impedance and otological examination were also consistent with the diagnosis of NSHL.

Fig. 1.

a Pedigree diagram of the four generations of FY-140 with ADNSHL. b Audiogram curves of the 4 affected members of FY140. Right ear tonal audiometric curves showing that the audiogram of III-1 exhibits a ‘cookie-bite’ pattern

Table 1.

Summary of the phenotypic information of the family members

Subjects	Gender	Age (years)		Pure-tone average (dBHL)		Audiogram shape	Degree of hearing loss
		At testing	At onset	Left	Right
I-1	Male	76	–	82	90	–	Severe
I-2	Female	72	–	Normal	Normal	–	Normal hearing
II-1	Male	62	–	Normal	Normal	–	Normal hearing
II-2	Female	57	42	77	98	Flat	Moderate–severe
II-3	Male	54	43	83	94	Flat	Severe
II-4	Female	51	–	Normal	Normal	–	Normal hearing
II-5	Male	53	–	Normal	Normal	–	Normal hearing
II-6	Female	44	37	75	71.25	Flat–sloping	Moderate–severe
III-1	Male	32	26	55	45	Cookie–bite	Moderate
III-2	Male	30	–	Normal	Normal	–	Normal hearing
III-3	Male	27	–	Normal	Normal	–	Normal hearing
III-4	Female	26	–	Normal	Normal	–	Normal hearing
III-5	Female	26	–	Normal	Normal	–	Normal hearing
IV-1	Male	4	–	Normal	Normal	–	Normal hearing
IV-2	Female	2	–	Normal	Normal	–	Normal hearing

Identification of novel CNV by TNGS

Patients II-2, III-1, and III-5 were subjected to targeted NGS of 147 deafness-related genes, and 8 SNPs and 1 CNV were detected. A novel CNV in exons 8–11 of the EYA4 gene and two previously identified variants (c.7630T > G, p. Leu2544Val and c.8257G > A p. Ala2753Thr) in the CDH23 gene were found in all three patients (Fig. 2A, B). The intrafamilial cosegregation of the variants and the hearing loss phenotype were confirmed by long-range PCR and Sanger sequencing in all family members.

Fig. 2.

Identification of a novel copy number variation in the EYA4 gene in a Chinese family. a Schematic diagram showing the position of the EYA4 gene deletion on chromosome 6. The red bar indicates the alignment position of the deletion. b Copy number of each exon calculated from the fluorescence peak ratios identified from the CNV analysis. c Scheme of the normal and inverted alleles. d Gap-PCR product of the distal breakpoint junction showing segregation with the phenotype in the family. e Sanger sequencing of the inverted allele by EYA4_Ex7_F and EYA4_In11_R covering the two breakpoints and a 10-bp insertion

We found that both variants of the CDH23 gene (NM_022124), c.7630T > G (p. Leu2544Val) in exon 52 and c.8257G > A (p. Ala2753Thr) in exon 54, were been carried by four patients (II-2, II-3, II-6 and III-1) and five healthy family members (I-2, III-2, III-3, III-5, IV-1 and IV-2) by Sanger sequencing. These four patients and five healthy family members are all heterozygous for both variants of the CDH23 gene. According to the pedigree diagram, the inheritance pattern of the hearing loss in this family was dominant. Both variants of the CDH23 gene did not co-segregate with the phenotype (BS4-ACMG).The genotype for each variant in the pedigree and the Sanger sequencing results of these family members are described in the supplemental material (Additional file 1: Fig. S1, Additional file 2: Fig. S2, Additional file 3: Fig. S3). We believe they are in cis-mutation and not in compound heterozygosity in each subject. Although they have been reported in a previous paper that detected both variants in a deafness patient from China, these variants did not appear in the general populations databases [26]. The minor allele frequency (MAF) of p. Leu2544Val variant is 0.000051 (East Asian) in GnomAD, while the p. Ala2753Thr variant is novel (PM2-ACMG). These variants were predicted as benign by silico pathogenicity prediction tools REVEL, MutationTaster, SIFT and Polyphen 2 (BP4-ACMG). According to ACMG standards and guidelines, both variants in the CDH23 gene are classified as PM2, BP4 and BS4, and the criteria for both alleles will be of uncertain significance (Table 2). In general, CDH23 gene mutations cause autosomal recessive non-syndromic hearing loss [4, 27]. Based on the ACMG classification and intrafamilial cosegregation analysis of these variants, we inferred that the c.7630T > G and c.8257G > A variants in the CDH23 gene may not be the cause of the disease in this family. In contrast, a CNV in the EYA4 gene was found only in the patients, and none of the healthy family members were carriers of this deletion (Fig. 2C). Therefore, this variant in the EYA4 gene could be considered the cause of the disease. Because patient I-1 died, we were unable to collect a sample of his DNA. We inferred that patients II-2, II-3 and II-6 inherited the heterozygotic variant from their father and that patient III-1 inherited the variant from patient II-2.

Table 2.

Identified CDH23 variant and in silico molecular genetic analysis

Gene name	Transcript accession number	cDNA change	Protein change	Position (GRCh38/hg18)	REVEL	MutationTaster	SIFT	Polyphen 2	ACMG Classification
CDH23	NM_0221 24;exon52	c.7630T > G	p.Leu2544Val	chr10:71803045	Benign (0.025)	Disease causing (0.999999989)	Benign (0.393)	Benign (0.002)	Uncertain Significance (PM2 + BP4 + BS4)
CDH23	NM_0221 24;exon56	c.8257G > A	p.Ala2753Thr	chr10:71807355	Benign (0.363)	Disease causing (0.999999778)	Benign (0.01)	Benign (0.032)	Uncertain Significance (PM2 + BP4 + BS4)

Verification of CNV by WGS

We performed WGS using genomic DNA from patient III-1 to further identify potential variants. We identified on average 3,585,580 SNPs and 732,397 indels in coding regions or introns. Then, Single-nucleotide variants (SNVs) and InDel from WGS data were filtered as follows: (1) variants with MAF below 0.01 in 1000Genomes, ExAC03 Asian population and gnomAD Asian population (2) coding/splicing variants (3) variants that are predicted to be likely pathogenic/pathogenic by any of the following software such as SIFT, POLYPhen V2, MutationTaster, Cadd, Dann, and dbscSNV, were considered as likely causal variants. CNV analysis was performed using the log2-ratio of read depth on each exon. Priority was given to variants found in deafness genes (annotated as deafness genes in one or more of the following databases: OMIM, HPO, HGMD, InterVar, HPO, MGI, ClinVar, ISCA and MalaCards). There were 64 variants predicted as candidates, including 47 SNVs, 3 indels, and 2 CNVs. With WGS, TNGS, Sanger sequencing, and cosegregation analysis, we identified a novel CNV that might cause disease.

The complex genomic rearrangement in the dataset encompassed a 17.4-kb deletion spanning exons 8 to 11 and a 10-base insertion (chr6: 133461414 insTTTGAATTTT). As shown in Fig. 2, the distal breakpoint mapped to chr6: 133461414 (GRCh38/hg38), and the proximal breakpoint mapped to chr6: 133478832 (GRCh38/hg38) (Fig. 2C). To validate the identified CNV, gap-PCR of the region spanning the breakpoint was performed. The primers Forward1 and Reverse amplify a gap-PCR product specific for the variant allele, whereas the primers Forward2 and Reverse amplify a product from the normal EYA4 gene. The 500-bp PCR product was obtained from all 4 affected individuals (II-2, II-3, II-6 and III-1) in the family but not from the unaffected family members (I-2, III-2, III-3, III-5, IV-1 and IV-2). The normal EYA4 gene product was found in all family members (Fig. 2D). The generated PCR products were subjected to Sanger sequencing, and a sequence analysis of the resultant amplicons confirmed the junction of the two breakpoints through the 10-bp insertion identified by WGS (Fig. 2E). This deletion has been submitted to LOVD under accession ID 00361730.

Discussion

CNVs are a common cause of hereditary hearing loss and are thought to play a role in nearly 20% of non-syndromic HL diagnoses [19]. In this study, we performed a comprehensive genetic analysis that included TNGS, WGS, gap-PCR and Sanger sequencing in a four-generation Chinese Han family with autosomal dominant NSHL. All the affected individuals in the family exhibited sensorineural deafness, which primarily affected low and mid-frequencies and had onset ages in the range of 26 to 43 years.

We identified a novel CNV deletion in exons 8–11 of the EYA4 gene with a 10 bp insertion. First, this variant cosegregated with NSHL symptoms in patients and was not detected in normal family members. Then, this CNV is predicted to affect the eyaHR domain. By interacting with members of the SIX and DACH protein families in a conserved network, the highly conserved C-terminal region of EYA4 (eyaHR) regulates embryonic development and follow-up functions after development of the mature organ of Corti. It regulates Na+/K+-ATPases and the development of mechanosensory cells of the inner ear [12]. Finally, we attempted to construct a three-dimensional structure of the CNV using the SWISS-MODEL software but failed because the structure was severely affected. These findings may also suggest that this novel CNV deletion is pathogenic in auditory function.

The hearing loss phenotype in the present family is similar to that reported for patients carrying EYA4 variants, i.e., late-onset, postlingual, progressive, and bilateral HL. In previous reports, flat-type hearing loss was observed in patients with truncating EYA4 variants. At onset, hearing impairment was usually mild and detected at mid-frequencies, resulting in an audiometric profile commonly referred to as a “cookie-bite” pattern. During its progression, hearing loss began to involve other frequencies. The progression rate of hearing loss caused by EYA4 was approximately 5.75 dB/year (95% CI 4.50–7.00 dB/year), which is relatively severe compared to POU4F3 and MYO6 gene mutation in ADNSHL patients [28, 29].

To date, variants in the EYA4 gene have been associated with HL in more than 50 ethnic groups worldwide. It was believed that EYA4 variants led to syndromic and non-syndromic NSHL, but EYA4 is not a frequently mutated gene in ADNSHL compared with other reported genes. The characteristics of all known EYA4 variants are summarized in Table 3. According to these variants, the severity of hearing loss was not significantly related to the types or locations of variants.

Table 3.

Summary of all known EYA4 variants and their hearing loss phenotypes

Variant type	Nucleotide Change	Exon/Intron	Amino Acid Change	Origin	Age at HL on set	HL degree	Audiogram profile	References
Splicing	c.84-2A > G	Intron 3		Chinese Indian	N/A	N/A	N/A	Chen et al. 2016 [36] Panigrahi et al. [37]
Missense	c.152C > T	Exon 4	p.Ser51Phe	America	N/A	N/A	N/A	Sloan-Heggen et al. [38]
Nonsense	c.160G > T	Exon 4	p.Glu54*	Spanish	42 years	Mild	MF/flat	Morín et al. [13]
Frameshift	c.222_223del	Exon 5	p.Val75Phefs*32	Japanese	61 years	Mild to moderate	HF/LF	Shinagawa et al. [9]
Frameshift	c.464delC	Exon 8	p.Pro155Glnfs*43	Swedish Dutch	N.A Childhood	N.A Moderate	N.A MF/HF	Neveling et al. [39] Van Beelen et al. [40]
Frameshift	c.498del	Exon 8	p.Thr167Leufs*31	Japanese	13 years	Mild	LF	Shinagawa et al. [9]
Missense	c.511G > C	Exon 8	p.Gly171Arg	Chinese	6–50 years	Mild to severe	HF/flat	Liu [15]
Nonsense	c.517C > T	Exon 8	p.Gln173*	Japanese	48 years	Moderate	Flat	Shinagawa et al. [9]
Missense	c.543C > G	Exon 8	p.Tyr181Ter	Chinese	Second to the fourth decade	Severe to profound	Flat	Mi et al. [32]
Frameshift	c.579_580insTACC	Exon 8	p.Asp194Tyrfs*52	Swedish	4–40 years	Mild to profound	N/A	Frykholm et al. [41]
	c.580 + 1G > A	Intron 8		Japanese	45 years	Moderate	Flat	Shinagawa et al. [9]
Frameshift	c.614dupA	Exon 9	p.Glu205Argfs*40	Chinese	20–40 years	Moderate to profound	HF/flat	Huang et al. [42]
Frameshift	c.781del	Exon 10	p.Thr261Argfs*34	Spanish	26–44 years	Mild to moderate	Gently downsloping	Morín et al. [13]
Missense	c.804G > C	Exon 10	p.Gln268His	Slovak	10–40 years	Moderate	Gently downsloping	Varga et al. [43]
Nonsense	c.863C > A	Exon 11	p.Ser288*	Korean Korean	N.A N.A	Moderate Moderate to severe	Reverse U-shaped Flat	Baek et al. [44] Kim et al. [10]
Missense	c.866C > T	Exon 11	p.Thr289Met	American	N.A	N.A	N.A	Miszalski-Jamka et al. [45]
Frameshift	c.910del	Exon 11	p.Ser305Leufs*15	Japanese	30 years	Severe	Flat	Shinagawa et al. [9]
Missense	c. 978C > G	Exon 12	p.Phe326Leu	Korean	N.A	Moderate	Down sloping	Choi et al. [46]
Nonsense	c.988C > T	Exon 12	p.Gln330*	Japanese	16 years	Moderate	Flat	Shinagawa et al. [9]
Frameshift	c.1026_1027dupAA	Exon 12	p.Thr343Lysfs*62	American	N.A	Moderate to profound	Flat/Gently sloping	Wayne et al. [12]
Frameshift	c.1048_1049dupAA	Exon 12	p.Arg352Profs*53	American	N.A	Moderate to severe	MF/HF	Makishima et al. [47]
Missense	c.1078C > A	Exon 12	p.Pro360Thr	Spanish	44 years	Mild to moderate	Gently downsloping	Morín et al. [13]
Missense	c.1107G > T	Exon 12	p.Glu369Asp	Spanish	10–11 years	Moderate to severe	Gently downsloping	Morín et al. [13]
Missense	c.1109G > A	Exon 13	p.Arg370His	Philippines	N.A	N.A	N.A	Truong et al. 2019 [48]
Missense	c.1109G > C	Exon 13	p.Arg370Pro	Japanese	30 years	Mild to moderate	MF	Shinagawa et al. [9]
Missense	c.1109G > C	Exon 13	p.Val371Met	Belgium	N.A	N.A	N.A	Sommen et al. [49]
Frameshift	c.1115_1118dup TTGT	Exon 13	p.Trp374Cysfs*6	Hungarian	N.A	N.A	N.A	Pfister et al. [50]
Missense	c.1122G > T	Exon 13	p.Trp374Cys	Australian	10–25 years	Mild to severe	Gently downsloping	Morín et al. [13]
Missense	c.1154C > T	Exon 13	p.Ser385Leu	Italian	N.A	Mild to profound	MF	Cesca et al. [51]
Nonsense	c.1177C > T	Exon 13	p.Gln393*	Korean Japanese	N.A 26 years	moderate	HF Flat	Kim [10] Shinagawa et al. [9]
Frameshift	c.1194del	Exon 14	p.Met401Trpfs*3	Korean	N.A	Moderate	Down sloping	Choi et al. [46]
Missense	c.1216G > C	Exon 14	p.Gly406Arg	Japanese	5 years	Moderate	Flat	Shinagawa et al. [9]
Missense	c.1223G > A	Exon 14	p.Arg408His	America	N.A	N.A	N.A	Miszalski-Jamka et al. [45]
Missense	c.1281G > A	Exon 14	p.Glu427Glu	Spanish	26 years	Moderate to profound	Flat	Morín et al. [13]
Splicing	c.1282-12T > A	Intron 14		Australian	N.A	Mild to profound	Flat	Hildebrand et al. [52]
Splicing	c.1282-1G > A	Intron 14		Spanish	12 years	Mild to moderate	MF/Flat	Morín et al. [13]
Missense	c.1301T > A	Exon 15	p.Ile434Lys	Chinese	8–38 years	Mild to severe	MF/flat	Tan et al. [53]
Splicing	c.1341-19T > A	Intron 15		Germany	N.A	N.A	N.A	Vona et al. [54]
Nonsense	c.1601C > G	Exon 17	p.Ser534*	Spanish	3–16 years	Moderate to severe	MF/Flat	Morín et al. [13]
Missense	c.1643C > G	Exon 18	p.Thr548Arg	Chinese	17–40 years	Mild to profound	N.A	Sun et al. [11]
Missense	c.1663G > C	Exon 18	p.Ala555Pro	Japanese	25 years	Moderate	N.A	Shinagawa et al. [9]
Splicing	c.1739-1G > A	Intron 18		America	50 years	N.A	N.A	Cirino et al. [55]
Nonsense	c.1759C > T	Exon 19	p.Arg587*	Belgian	6–40 years	Mild to moderate	N.A	Wayne et al. [12]
Frameshift	c.1790del	Exon 19	p.Val597Glyfs*4	Japanese	35 years	Moderate	Flat	Iwasa et al. [56]
Missense	c.1810G > T	Exon 19	p.Gly604Cys	Swedish Dutch	N.A	N.A	N.A	Neveling et al. [39] Van Beelen et al. [40]
Nonsense	c.1834A > T	Exon 19	p.Lys612*	Chinese	27 years	Moderate	Gently downsloping	Hu et al. [16]
Missense	c.1855T > G	Exon 20	p.Trp619Gly	Chinese	N.A	N.A	N.A	Xiao et al. [57]
CNV	Deletion 7689 bp (Ex7 to Ex11)			Japanese	25 years	Moderate to severe	LF/HF	Shinagawa et al. [9]
CNV	Deletion 9.5 Mb (Ex4 to Ex 20)			Japanese	13 years	Severe	LF/HF	Shinagawa et al. [9]
CNV	Deletion 2747 bp (Ex15 to Ex17)			Spanish	8 years	Moderate	Flat	Morín et al. [13]
CNV	Deletion 9 Mb at 6q23.1–24.1 (Ex4–20)		p.Asp194Glyfs*30	Polish	N.A	N.A	N.A	Dutrannoy et al. [30]
CNV	Deletion 4846 pb incl. intron 9, exon 10 and partial intron 10 c.581_804del (In9, Ex10, part of In10)				N.A	N.A	N.A	Schönberger et al. [34]
CNV	Deletion 10.4 Mb promoter and exon 1,2 (Ex1–2)			Japanese	20-month-old	Moderate to severe	MF/flat	Abe et al. [31]
CNV	Deletion 3.7 MB in 6q23.1q23.2 (Ex1–20)			Italian	12 years	N.A	N.A	Gana et al. [58]
CNV	Deletion 12,835 bp (Ex6–10)			Japanese	23 years	Mild to sereve	LF/HF/Flat	Ishino et al. [14]
CNV	Deletion 17.4 kb and 10 bp insertion (Ex8–11)			Chinese	26–42 years	Moderate–severe	Flat	This work

Several CNVs in EYA4 have been linked to deafness (Fig. 3). One CNV disrupts the EYA4 gene and spares only exons 1–3 from the deletion [30]. The deleted sequence of the promoter and the first two exons was previously identified in a Japanese boy [31]. A deletion of four exons and a deletion spanning exons 4–20 have been reported to cause severe ADNSHL in Japanese individuals [9]. In addition, the EYA4 variant reportedly causes dilated cardiomyopathy accompanying NSHL in a single large family. In this family, a 4846-bp genomic deletion that resulted in loss of the EYA domain (eyaHR) and part of the variable region (eyaVR) was detected [32]. A heterozygous deletion of 2747 bp represented a copy variant loss encompassing exon 15 to exon 17 [13]. Additionally, in Japan, a novel hemizygous indel in the EYA4 gene was predicted to be p. (Val124_Pro323del) [14]. We now add a genomic rearrangement consisting of a deletion and a 10-bp insertion to this list (Fig. 3, Table 3).

Fig. 3.

Overview of the CNVs identified in this study and those previously identified in EYA4

The EYA4 gene is widely distributed in the inner ear, which includes otic vesicles, the Reissner membrane and the sensory epithelia of the vestibular system and Corti [12, 33]. Although the pathogenic mechanism of ADNSHL associated with EYA4 variants remains to be further investigated, haploinsufficiency is generally thought of as the major mechanism. Many reports indicate that EYA4 participates in important pathways in cardiac tissue. The physiological level of Eya4 phosphatase activity is thought to participate in normal cardiac gene regulation. Instead of most DCM genes that encode structural proteins, EYA4 is a transcriptional coactivator. Large deletions comprising the variable domain are most likely to affect cardiac functions. A patient carrying a de novo 9 MB interstitial deletion that disrupts the gene EYA4 presented a patent ductus arteriosus and aortic insufficiency. A family with a 4846-bp deletion was associated with DCM as well as NSHL. In the other 2 unrelated families, polymorphic loci on chromosome 6q23 to 24 were associated with DCM and NSHL. [30, 34]. For that reason, we performed electrocardiograms for all family members. None of the members exhibited a cardiac phenotype, and electrocardiograms showed no abnormalities. Including our work, many studies indicated that the genotype–phenotype correlation of large deletions in EYA4 and dilated cardiomyopathy was not very obvious [14]. One reason may be that previously reported cardiopathy was caused by other variants in the large deleted regions. Another reason is that the defect in a contiguous gene could account for the cardiac defects [34]. More experiments, including detecting EYA4 levels in nuclear and cytoplasmic components, may provide evidence for this theory. Increasing the yield of genetic testing among patients with both NSHL and DCM may allow for better detection of the EYA4 gene and cardiac pathology [35].

Conclusions

A novel CNV deletion at 6q23 in exons 8–11 of EYA4 in a Chinese ADNSHL family was identified by WGS and Sanger sequencing. The phenotype of the family differed from that of previously reported pedigrees with CNV deletion of EYA4 variants. The phenotypes also differed between individuals with the same variant in the same family. Our results highlight the complexity of the EYA4 genotype and phenotype.

Author contributions

WZ, JS: original draft preparation, conducting experiments. JY and YY reviewed the manuscript. LG, MM, BT: conceptualization, supervision, methodology, reviewing and editing manuscript. All authors read and approved the final manuscript.

Funding

This project has received funding from the Natural Science Foundation of Shanghai (No 19ZR1408700), the Shanghai 2020 Science and Technology Innovation Action Plan One Belt One Road International Cooperation Project (20410740600) and the Clinical Research Plan of SHDC (SHDC2020CR1049B).

Availability of data and materials

The authors are not able to share the clinical data due to full anonymisation of the data is very difficult. The CNV in our study was called based on the human assembly GRCh38 (https://hgdownload.soe.ucsc.edu/downloads.html#human). This CNV has been submitted to LOVD under accession ID 00361730. All sequencing data used to support the findings of this study are available from the corresponding author upon request.

Declarations

Ethics approval and consent to participate

The study was conducted in accordance with the principles of the Declaration of Helsinki. Approval for the study was obtained from the Ethics Committee of the Eye & ENT Hospital, Fudan University for Human Studies. Written informed consent was obtained from the participants or the parents of minors.

Consent for publication

Not applicable.

Competing interests

None declared.