Fig. 3. Blood pressure and plasma membrane GluA1 IGS labeling in TNFR1-labeled dendritic profiles of PVN neurons following AngII infusion in male mice.

Male mice infused with either Veh or AngII did not differ in SBP prior to pump implantation (Day 0). In contrast to mice treated with vehicle, mice receiving AngII showed increased SBP on the last day of blood pressure recording (A). The partitioning ratio of plasma membrane GluA1 IGS in small-size dendritic profiles of PVN neurons was higher in male mice infused with AngII compared to Veh (B). In contrast, there were no differences in the partitioning ratio of plasma membrane GluA1 in large dendritic profiles (C) or in dendrites collapsed across all profile sizes (D) in male mice from each treatment group. Light micrograph illustrating GluA1 labeling in the dorsal hypothalamus of a vehicle-infused mouse (E). The location of the PVN is indicated in the dashed area. Electron micrograph illustrating size-regions in a longitudinally-sectioned dendritic profile from a male mouse infused with vehicle (Veh, F) where GluA1 immunogold-silver (IGS) labeling is present in the cytosol (arrows). Large (double chevron) and small (single chevron) regions of the dendritic profile are illustrated. Cross-sectionally labeled dendrites from mice infused with Veh (G) or AngII (H). In the Veh condition, IGS labeling for GluA1 is present in the cytoplasm (arrows). Labeling for GluA1 is shown near the plasma membrane (arrowhead) of a dendritic profile from an AngII-treated mouse. PM/Total: Plasma membrane labeling/Total labeling. A: * p< 0.05 SBP Veh versus AngII day 13; B: * p< 0.05 Veh versus AngII GluA1 IGS PM/Total in small dendrites. Lines in A-D are medians which are overlain with data points representing means for each animal. Scale bars: E: 250 μm; F: 1 μm; G-H: 500 nm