Figure 3.

The genetic absence of CD38 inhibited the antigen persistent stimulation-induced T dysfunction

In this figure, all representative contour plots of indicated protein expression were placed to the left, and statistical results were on the right. All error bars indicate Mean ± SD (n = 3), Student’s t test, ∗∗∗∗: p < 0.0001; ∗∗∗: p < 0.001; ∗∗: p < 0.01; ∗: p < 0.05; ns: not significant.

(A and B) Activated WT or CD38 knockout OT-1 cells were treated with antigen chronic stimulation as shown in Figure S1D, and then following flow cytometry analysis of PD-1, TIM-3, and Ly108 expression. Percentages of PD-1⁺TIM-3⁺ and Ly108⁻TIM-3⁺ were compared.

(C) The expression and MFI of transcript factors TCF-1 and TOX in WT or CD38 knockout OT-1 cells under continuous OVA peptides treatment or not were analyzed by flow cytometry.

(D and E) The ability of cytokine production of antigen persistent stimulated-WT or Cd38^−/− OT-1 cells were analyzed by comparing the percentages of TNFα⁺IFNγ⁺ or IL-2⁺ frequencies in the indicated cells.

(F) The mitochondria redox levels in OT-1 cells with indicated treatment or not were labeled by Mito-SOX, and then percentages of Mito-SOX⁺ in these four groups were compared.

(G) γ-H2AX was utilized as a marker to reflect the DNA damage levels of indicated cells under antigen continuous stimulation or not. Values indicate the MFI of γ-H2AX in OT-1 cells.