| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| GATA6 | Cell Signaling | 5851 RRID:AB_10705521 |
| GATA4 | Santa Cruz | SC-1237 RRID:AB_2108747 |
| SOX17 | RnD | AF1924 RRID:AB_355060 |
| FOXA2 | RnD | AF2400 RRID:AB_2294104 |
| EOMES | Cell Signaling | 66325 RRID: Unknown |
| EOMES (RIME analysis) | Abcam | Ab23345 RRID: AB_778267 |
| FLAG | Sigma-Aldrich | F1804 RRID:AB_262044 |
| Normal Mouse IgG | Sigma-Aldrich | 12-371 RRID:AB_145840 |
| β-Actin | Sigma-Aldrich | A1978 RRID:AB_476692 |
| Protein G Dynabeads | ThermoFisher Scientific | 10004D |
| Bacterial and virus strains | ||
| pzbFGF BL21 Star E. coli | (Ludwig et al., 2006) | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| mTesR | (Ludwig et al., 2006) | N/A |
| E-cadherin | (Nagaoka et al., 2010) | N/A |
| RPMI 1640 | ThermoFisher Scientific | 22400089 |
| Geltrex | ThermoFisher Scientific | A1413302 |
| B-27 Supplement (50x), minus insulin | ThermoFisher Scientific | A1895601 |
| Activin A Recombinant Human Protein | ThermoFisher Scientific | PHC9563 |
| FGF-Basic (AA 10-155) Recombinant Human Protein | ThermoFisher Scientific | PHG0023 |
| Purified recombinant zebrafish FGF-Basic | (Ludwig et al., 2006) | N/A |
| BMP4 Recombinant Human Protein | ThermoFisher Scientific | PHC9533 |
| Formaldehyde 16%(w/v) | ThermoFisher Scientific | 28906 |
| Puromycin | Sigma-Aldrich | P9620 |
| Doxycycline hyclate | Sigma-Aldrich | D9891 |
| ROCK inhibitor Y27632 | StemRD | 146986-50-7 |
| DAPI | Sigma-Aldrich | D1388 |
| Critical commercial assays | ||
| RNeasy mini Kit | QIAGEN | 74106 |
| TURBO DNA-freeTM Kit | ThermoFisher/Ambion | AM1907 |
| M-MLV Reverse Transcriptase | ThermoFisher/Invitrogen | 28025-013 |
| TaqMan Gene Expression | ThermoFisher/Applied Biosystems | 4369016 |
| Pierce BCA protein assay kit | ThermoFisher Scientific | 23227 |
| Pierce co-immunoprecipitation kit | ThermoFisher Scientific | 87787 |
| SimpleChIP Plus Enzymatic ChIP Kit | Cell Signaling | 9005 |
| Transcription Factor Staining Buffer Set | ThermoFisher Scientific/eBioscience | 00-5523-00 |
| Experimental models: cell lines | ||
| IPSC-K3 | (Si-Tayeb et al., 2010b) | N/A |
| IPSC-K3 GATA6Ex4Δ1/Δ2;indGATA6 | (Heslop et al., 2021) | N/A |
| Oligonucleotides | ||
| GATA6 | Fwd: TTCGTTTCCTGGTTTGAATTCC Probe: TCATAGCAAGTGGTCTGGGCACC Rev: TGCAATGCTTGTGGACTCTAC |
Integrated DNA Technologies |
| EOMES | Fwd: GTGGCAAAGCCGACAATAAC Probe: CCGAATGAAATCTCCTGTCTCA Rev: ACCCAGAGTCTCCTAATACTGGTTCCC |
Integrated DNA Technologies |
| RPL13a | Fwd: GCCTTCACAGCGTACGA Probe: AGCAGTACCTGTTTAGCCACGATGG Rev: CGAAGATGGCGGAGGTG |
Integrated DNA Technologies |
| Software and algorithms | ||
| Graphpad with PRISM | https://www.graphpad.com/ | Version 8.4.2 |
| FlowJo | https://www.flowjo.com/ | Version 10.5.0 |
| ImageJ | https://imagej.nih.gov | Version 1.53q |
| EaSEQ | (Lerdrup et al., 2016); https://easeq.net/ | Version 1.111 |
| FastQC | https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ | Version 0.11.7 |
| Partek Flow | https://www.partek.com/partek-flow/ | Version 9.0.20.0526 |
| STAR | Partek Flow | Version 2.6.1d |
| BOWTIE2 | Partek Flow | Version 2.2.5 |
| MACS2 | Partek Flow | Version 2.1.1 |
| DiffBind | (Ross-Innes et al., 2012); https://bioconductor.org/packages/DiffBind/ | Version 3.13 |
| PANTHER Classification system | (Mi et al., 2013) http://pantherdb.org | Version 17.0 |
| HOMER | (Heinz et al., 2010); http://homer.ucsd.edu/homer/ | Version 4.10 |
| Other | ||
| ATAC-seq data | (Heslop et al., 2021) | GSE156021 |
| RNA-seq data | (Heslop et al., 2021) | GSE156021 |
| CHIP-seq: GATA6 | (Heslop et al., 2021) | GSE156021 |
| CHIP-seq: EOMES | (Heslop et al., 2021) | GSE156021 |
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