Figure 1. CRISPR/Cas9 editing of Stmn2.

(A) Diagram of breeding strategy to generate Stmn2 F₀, Rosa26 gRNA, and Stmn2 F₂ mice. (B) Schematic representation of the STMN2 locus in which gRNAs were targeted to exons 2 and 4 to create a predicted 13 Kb deletion. Primers were designed to flank the region of the deletion region to confirm the presence or absence of mutations. (C) PCR genotyping of F₂ Stmn2 mice exhibiting WT (+/+), heterozygous (+/−), and homozygous (−/−) mutations. (D) F₂ Stmn2 brain mRNA levels flanking Exons 1 to 3 (left) and Exons 4 to 5 (right). (E) Spinal cord and brain Stmn2 protein levels from Western blots including the housekeeping protein GAPDH. **** p < 0.0001. In all figures: results are shown as a mean with error bars calculated as standard deviation. Detailed information (average, SD, n and detailed statistics) is shown in STAR Methods.