Figure 10. Codon adaptation and in silico cloning of the chimeric protein.

(A) Codon adaptation result of vaccine construct V1 predicted by JCat tool predicting that the optimized codon sequence has a length of 978 nucleotides and its CAI (codon adaptation index) was predicted to be 1.0, with an average of 41.21% GC for the adapted sequence. (B) Final protein in-silico restriction cloning into pET28a (+) vector. Here, the red portion represents the gene sequence of the designed vaccine, and the black portion denotes the backbone of the vector. The DNA sequence is inserted into the MCS region of the cloning vector.