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. 2022 May 23;11(1):28. doi: 10.1038/s41389-022-00402-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Growth curve describing the effect of ZDHHC4 expression on TMZ inhibition in GBM cells. SF126 cells with overexpression or knockout of ZDHHC4 were treated with different concentrations of TMZ. B Representative image of colony formation in the SF126 cell lines 14 days after different treatments. For the control group, DMSO and TMZ were used. For the pLKO.1-ZDHHC4 group, DMSO, TMZ, and TMZ + Colivelin were used. For the pLVX-ZDHHC4 group, DMSO, TMZ, and TMZ + Stattic were used. Colonies were stained using crystal violet. C The number of clones stained by crystal violet in each dish in Figure B is calculated. Con-DMSO group was set at 100%. D ABE analysis of GSK3β palmitoylation in SF126 and SF126R cells. E Western blot analysis of GSK3β and STAT3 phosphorylation in SF126 and SF126R cells. F Immunoprecipitation analysis of the STAT3 binding capacity of GSK3β and EZH2 in SF126 and SF126R cells. G CCK-8 assay was used to detect the effect of GSK3β (C14A) mutant on TMZ killing SF126R cells. Data are shown as means ± SD (n = 3). P-values were determined by two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001.