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. 2022 May 5;25(6):104344. doi: 10.1016/j.isci.2022.104344

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Zebrafish embryos assemble embryonic RNA granules consisting of sox19b or nanog mRNA and mouse embryos assemble those consisting of Pou5f1/Oct4 mRNA

(A) FISH analyses of sox19b (upper) or nanog (lower) mRNA (green) in zebrafish embryos at 0, 3, and 6 hpf. DNA is shown in blue. Similar results were obtained from two independent experiments.

(B) Double FISH analysis of pou5f3 (green) and sox19b (red) (upper) or nanog (red) (lower) mRNAs in zebrafish embryos at 0 and 3 hpf. DNA is shown in blue. Similar results were obtained from two independent experiments.

(C) Immunostaining of Pou5f1/Oct4 (left) in mouse blastocysts. A bright-field image with DNA staining in blue is shown on the right. ICM, inner cell mass.

(D) FISH analysis of Pou5f1/Oct4 mRNA (green) in mouse blastocysts. An enlarged image of the boxed region is shown on the right. DNA is shown in blue. Similar results were obtained from five independent experiments.

(E) Simultaneous detection of Pou5f1/Oct4 mRNA (green) and newly synthesized peptides (red) in mouse blastocysts. DNA is shown in blue. Arrows indicate the regions where both signals overlapped. Bars, (A and B) 20 μm, (C and D) 10 μm, (E) 2 μm.