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. 2022 May 13;25(6):104405. doi: 10.1016/j.isci.2022.104405

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

BMP-2 stimulated the proliferation and differentiation of NPMSCs

(A–C) The protein levels of COL 2A1 and ACAN were assayed by western blotting (A), and the relative quantitative data (B and C) were calculated. GAPDH was used as an internal control. The results showed that the protein expression of COL 2A1 and ACAN at 200 ng/mL rhBMP-2 was higher than that in the other groups. Results are presented as the mean ± SD of three independent experiments (∗p < 0.05, ∗∗∗p < 0.001).

(D and E) BMP-2 was knocked down using a silencing agent. The reagent effectively reduced the expression of BMP-2 protein in the cells. Results are presented as the mean ± SD of three independent experiments (∗∗∗p < 0.001).

(F–H) The protein expression of COL 2A1 and ACAN was significantly decreased after BMP-2 knockdown under PMS. Results are presented as the mean ± SD of three independent experiments (∗∗∗p < 0.001).

(I–J) Representative images of immunofluorescence staining for BMP-2 were obtained; and the relative fluorescence intensity in each group was calculated. Results are presented as the mean ± SD of three independent experiments (∗∗∗p < 0.001).