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. 2022 Apr 28;10(5):1016. doi: 10.3390/biomedicines10051016

Figure 4.

Figure 4

GSK’547 protects macrophages from LPS/zVAD-fmk-induced necroptosis. Bone marrow-derived macrophages (BMDMs) were isolated from wild-type mice and treated with 50 ng/mL LPS and 20 µM zVAD-fmk in the presence or absence of GSK’547 for 18 h. (A) Western blot analysis of necroptosis proteins (MLKL, RIPK3) on cell lysates. (B) Cell death was measured using propidium iodide (PI) labeling. *** p < 0.001 vs. 0 µM GSK’547 (one-way ANOVA followed by Dunnett’s post hoc test, n = 5 independent experiments).