Figure 4.

17-OHPC progesterone protects against fetal wastage

(A) Number of FOXP3+ and CD4+ T cells with I-A^b:2W1S_55-64 specificity, percent FOXP3+ among CD4+ T cells with I-A^b:2W1S_55-64 specificity, and CTLA-4 gMFI among 2W1S-specific FOXP3+ cells in the spleen and pooled peripheral lymph nodes 48 h following administration of 17-OHPC (open) or vehicle (filled) in C57BL/6 female mice mid-gestation (E11.5) during allogeneic pregnancy sired by Balb/c-2W1S/OVA (H-2^d) male mice.

(B) Percent fetal wastage, number of live pups in utero, and total concepti for 17-OHPC (open black) or vehicle-treated (closed black) Foxp3^DTR/WT female mice five days after initiating daily diphtheria toxin (DT) treatment during allogeneic pregnancy sired by Balb/c-2W1S/OVA (H-2^d) male mice.

(C) Percent and number of FOXP3+ Tregs with I-A^b:2W1S_55-68 specificity one day after initiating DT treatment for groups described in panel b.

(D) Number of H-2K^b:OVA_257-264-specific CD8+ T cells in the spleen and pooled peripheral lymph nodes or decidua five days after initiation of DT for groups described in panel B.

(E) Percent fetal wastage, number of live pups in utero, and total concepti for 17-OHPC-treated FOXP3-PRKO (open) or control FOXP3-PRKO (closed) mice. Data are from at least three independent experiments, each with similar results, with each point representing data from an individual mouse. Bar, mean ± SEM. ∗p < 0.05, ∗∗∗p < 0.005, ∗∗∗∗p < 0.001.