Table 2.
Antigens and commercial tests for the serological diagnosis of alveolar and cystic echinococcosis used in this study.
| ELISA */Commercial Test ** | Origin | Remarks | Citation or Material & Methods |
|---|---|---|---|
| EmVF CH/KG * | E. multilocularis | in vitro vesicle fluid | Schweiger et al., 2011 [20] |
| EmVC CH/KG * | E. multilocularis | in vitro vesicle crude antigen | 2.4.1 Vesicle crude antigens |
| EmP CH/KG* | E. multilocularis | protoscolex crude antigen from Meriones | Schweiger et al., 2011 [20] |
| Em2G11 CH/KG* | E. multilocularis | affinity-purified Em2G11 antigen from EmVC | Schweiger et al., 2011 [20] |
| mAb EmG3-EmVC CH/KG* | E. multilocularis | on-plate purification with monoclonal antibody | 2.4.2 On-plate purification |
| recEm18 * | recombinant | recombinant Em18 antigen | Schweiger et al., 2011 [20] |
| recEm95 * | recombinant | recombinant Em95 antigen | Gauci et al., 2002 [35] |
| EgVC * | E. granulosus s.s. | in vitro vesicle crude antigen | 2.4.1 Vesicle crude antigens |
| mAb EmG3-EgVC * | E. granulosus s.s. | on-plate purification with monoclonal antibody | 2.4.2 On-plate purification |
| EgCF * | E. granulosus s.s. | cyst fluid from sheep | Schweiger et al., 2011 [20] |
| EgP * | E. granulosus s.s. | protoscolex crude antigen from sheep | Schweiger et al., 2011 [20] |
| EgAgB * | E. granulosus s.s. | Antigen B from cyst fluid from sheep | Schweiger et al., 2011 [20] |
| recEg2B2 * | recombinant | recombinant 2B2 antigen | Hernández-González et al., 2012 [38] |
| Western blot-species ** | EUROIMMUN AG | for species diagnosis (AE vs. CE) | 2.4.4 Commercial Tests |
| Western blot-genus ** | EUROIMMUN AG | for genus diagnosis (AE or CE) | 2.4.4 Commercial Tests |
| EmVF-ELISA ** | EUROIMMUN AG | E. multilocularis vesicle fluid ELISA | 2.4.4 Commercial Tests |
| EgP-IFAT ** | EUROIMMUN AG | E. granulosus s.s. protoscolices IFAT | 2.4.4 Commercial Tests |
| VectorBest-ELISA ** | VectorBest | Echinococcus-IgG-EIA-BEST | 2.4.4 Commercial Tests |
* All antigens were evaluated with ELISA by direct coating or by on-plate purification with monoclonal antibodies (mAb). ** Commercial tests: EUROIMMUN (EUROLINE-Western blot, EmVF-ELISA, EgP-IFAT), VectorBest (VectorBest-ELISA). CH/KG These antigens were produced with an Asian and a European isolate of E. multilocularis.