Table 2.

Differentially expressed genes in the liver of broiler chickens as influenced by treatment groups ¹.

Treatments	Gene Symbol	Gene Description	Expression Level	log2FoldChange	p Value
B vs. A	ALDH1L2	aldehyde dehydrogenase 1 family member L2	Down	−0.6	<0.01
C vs. A	BTN1A1	butyrophilin subfamily 1 member A1-like	Up	0.4	0.02
D vs. A	AVD	Avidin	Up	0.5	<0.01
E vs. A	ST8SIA6	ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 6	Up	0.6	<0.01
	CUBN	Cubilin	Down	−0.7	0.01
F vs. A	ALDH1L2	aldehyde dehydrogenase 1 family member L2	Down	−0.6	0.01

¹ Treatments include (A) negative control treatment—chicks fed a basal corn-soybean meal-wheat-based diet; (B) in-feed antibiotics-chicks fed NC + 0.05% bacitracin methylene disalicylate; (C) in-water essential oil—chicks supplied the essential oil via the water route at the recommended dosage of 250 mL/1000 L of drinking water; (D) in ovo saline treatment—eggs injected with 0.2 mL of physiological saline (0.9% NaCl); (E) in ovo essential oil treatment—eggs injected with 0.2 mL of a saline + essential oil blend mixture at a dilution ratio of 2:1; (F) in ovo + in-water essential oil treatment-chicks offered the essential oil blend via the in ovo and in water route, successively. Each comparison is specified in the format “B vs. A”, where group B is compared to group A, with group A being the denominator for the comparison. Liver tissues (50–100 mg) were sampled from 8 replicate birds/treatment (independent of sex) using 1 mL TRIzol™ (Qiagen, Hilden, Germany).