Figure 1.

(a–c), Cell lines were prophylactically treated with probenecid 24 h prior to infection of RSV A2, RSV B1, or Memphis-37. Probenecid prophylaxis significantly (**** p < 0.0001) inhibited the virus replication in Vero E6 cells, HEp-2 cells, and NHBE cells compared to control (DMSO only). Viral titers were determined by plaque assay which has a limit of detection of 1 × 10² PFU/mL. The IC₅₀ and IC₉₀ values are shown in Table 1. (d–f), Cell lines were treated with probenecid 24h after infection of RSV A2, RSV B1, or Memphis-37. Treatment significantly (**** p < 0.0001) inhibited the virus replication in Vero E6 cells, HEp-2 cells, and NHBE cells compared to control (DMSO only). Viral titers were determined by plaque assay. The IC₅₀ and IC₉₀ values are shown in Table 1. For RSV B1 the IC₉₀ values are not available as the virus was not reduced by 90%.