Table 1.
Strains and plasmids used in this study.
| Strains/Plasmids | Characteristics * | Reference or Source |
|---|---|---|
| Acidovorax citrulli | ||
| M6 | Wild-type strain; ApR | [6] |
| M6-lip1- | M6 Tn5 mutant disrupted in gene APS58_1588 (lip1) by the insertion of EZ-Tn5 transposon; ApR, KmR | This study |
| M6-comp-lip1 | M6 lip1- mutant carrying pBBRlip1 (lip1 complemented strain); ApR, KmR, GmR | This study |
| M6-comp-ompW | M6 lip1- mutant carrying pBBRompW; ApR, KmR, GmR | This study |
| M6-comp-lip1-HA | M6 lip1- mutant carrying pBBRlip1-HA; ApR, KmR, GmR | This study |
| M6-comp-lip135-383-HA | M6 lip1- mutant carrying pBBRlip135-383-HA; ApR, KmR, GmR | This study |
| AAC00-1 | Wild-type strain; ApR | [4] |
| AAC00-1-lip1-HA | AAC00-1 carrying pBBRlip1-HA; ApR, GmR | This study |
| AAC00-1-T2SS- | AAC00-1 mutant impaired in type II secretion (double ΔgspG1/ΔgspG2 mutant); ApR | [27] |
| AAC00-1-T2SS--lip1-HA | AAC00-1-T2SS- carrying pBBRlip1-HA; ApR, GmR | This study |
| Escherichia coli | ||
| S17-1 λ pir | λ lysogenic S17-1 derivative producing π protein for replication of plasmids carrying oriR6K; recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 λ– pir |
[28] |
| Plasmids | ||
| pUC-4K | pUC4 derivative (pMB1 ori), containing the KmR gene from Tn903 that was used to generate the EZ-Tn5 transposon for mutagenesis; ApR, KmR | [29] |
| pMOD-3<R6Kγori/MCS> | transposon construction vector; ApR | Epicentre, Madison, WI, USA |
| pBBR1-MCS-5 | broad host range vector; GmR | [30] |
| pBBRlip1 | pBBR1-MCS-5 containing the promotor region (250-bp) of the lip1-ompW operon followed by the lip1 open reading frame (ORF) from M6; GmR | This study |
| pBBRompW | pBBR1-MCS-5 containing the promotor region (250-bp) of the lip1-ompW operon followed by the ompW ORF from M6; GmR | This study |
| pBBRlip1-HA | pBBR1-MCS-5 containing the lip1 ORF fused to the HA epitope at its C-terminus, under the control of the lac constitutive promoter; GmR | This study |
| pBBRlip135-383-HA | pBBR1-MCS-5 containing the lip1 ORF without the N-terminal signal peptide (first 102 bp of the ORF) fused to the HA epitope at its C-terminus, under the control of the lac constitutive promoter; GmR | This study |
* KmR, ApR and GmR indicate kanamycin, ampicillin and gentamicin resistance, respectively.