TABLE 2.
Characteristics of specific primers for cry9 genes
| Primer paira | Sequence of primersb | Gene recognized | Positionsc | Product size (bp) |
|---|---|---|---|---|
| EB-9A(d) | GGTTCACTTACATTGCCGGTTAGC | cry9A | 1581–1604 | 1,547 |
| Un9(r) | GTTTGAGCcGCTTCACAGCAATCC | 3104–3127 | ||
| EB-9B(d) | GCAAATGCATTTAGCGCTGGTCAA | cry9B | 1925–1948 | 701 |
| Un9(r) | GTTTGAGCcGCTTCACAGCAATCC | 2602–2625 | ||
| EB-9C(d) | CCACCAGATGAAAGTACCGGAAG | cry9C | 3473–3495 | 1,232 |
| Un9(r) | GTTTGAGCCGCTTCACAGCAATCC | 4681–4704 | ||
| EB-9D(d) | GCAATAAGGGTGTCGGTCACTGG | cry9D | 1754–1776 | 938 |
| Un9(r) | GTTTGAGCCGCTTCACAGCAATCC | 2668–2691 |
a
(d) and (r), direct and reverse primers, respectively.
b
Bases that do not match appropriate sequences are shown in lowercase letters.
c
Starting from the first base of the sequence (of the respective cry gene) in the GenBank database.