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. 2022 May 18;25(6):104426. doi: 10.1016/j.isci.2022.104426

Figure 6.

Figure 6

Increased infiltration of CCR10+ T cells in mucosal sites of CCL27−/− mice

All CCL27−/− and control CCL27+/+ littermate mice carry a CCR10-KO/EGFP-KI allele (CCR10+/EGFP) for the purpose of reporting CCR10 expression with EGFP.

(A) Percentages of CCR10(EGFP)+ subsets of T cells and ILCs in the spleen and blood of adult CCL27+/+ and CCL27−/− mice. N = 9 mice of each genotype for blood data, 12 for the spleen ILCs, CD8+, CD4+, γδ T cell data and seven for the spleen Treg data.

(B and C) FC analysis of gated populations of T cells and ILCs for their CCR10(EGFP) expression (B) and average percentages of CCR10(EGFP)+ subsets of T cells and ILCs (C) in lungs of CCL27+/+ and CCL27−/− mice. N = 4 each.

(D and E) FC analysis of gated populations of T cells and ILCs for their CCR10(EGFP) expression (D) and average percentages of CCR10(EGFP)+ subsets of T cells and ILCs (E) in uterus of female CCL27+/+ and CCL27−/− mice.

(F) Total numbers of CD45+ immune cells and different subsets of CCR10(EGFP)+ T cells and ILCs recovered from the uterus of female WT and CCL27−/− mice. N = 8 CCL27+/+ and seven CCL27−/− mice for the panels D-E. Statistical analysis by one tailed paired t-test. Significance: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.