Abstract
The increasing morbidity and mortality of type 2 diabetic mellitus (T2DM) patients with ischemic heart disease (IHD) highlight an urgent need to identify early biomarkers, which would help to predict individual risk of development of IHD. Here, we postulate that circulating serum-derived micro RNAs (miRNAs) may serve as potential biomarkers for early IHD diagnosis and support the identification of diabetic individuals with a predisposition to undergo IHD. We obtained serum samples from T2DM patients either with IHD or IHD-free and analysed the expression levels of 798 miRNAs using the NanoString nCounter technology platform. The prediction of the putative miRNAs targets was performed using the Ingenuity Pathway Analysis (IPA) software. Gene Ontology (GO) analysis was used to identify the biological function and signalling pathways associated with miRNA target genes. Hub genes of protein-protein interaction (PPI) network were identified by STRING database and Cytotoscape tool. Receiver operating characteristic (ROC) analysis was used to assess the diagnostic value of identified miRNAs. Real-time quantitative polymerase chain reaction (qRT-PCR) was used for nCounter platform data validation. Our data showed that six miRNAs (miR-615-3p, miR-3147, miR-1224-5p, miR-5196-3p, miR-6732-3p, and miR-548b-3p) were significantly upregulated in T2DM IHD patients compared to T2DM patients without IHD. Further analysis indicated that 489 putative target genes mainly affected the endothelin-1 signalling pathway, glucocorticoid biosynthesis, and apelin cardiomyocyte signalling pathway. All tested miRNAs showed high diagnostic value (AUC = 0.779 - 0.877). Taken together, our research suggests that circulating miRNAs might have a crucial role in the development of IHD in diabetic patients and may be used as a potential biomarker for early diagnosis.
Keywords: miRNA, ischemic heart disease, diabetes, miRNA profiling, biomarker
Introduction
Diabetes mellitus is a group of endocrine and metabolic disorders characterised by insufficient insulin secretion to maintain the right blood glucose level. According to the International Diabetes Federation, 463 million people had diabetes in 2019, and this number is expected to rise in the following year’s (1). Several forms of diabetes are distinguished; however, type 2 diabetes mellitus (T2DM) is the most common and accounts for over 90% of cases (2). T2DM is characterised by cell resistance to the normal concentration of insulin circulating in the blood. With the progression of the disease, pancreatic β-cell may also become dysfunctional and inevitably stop producing insulin (3). The most common long-term complications of diabetes is ischemic heart disease (IHD) (4, 5), and it is well known as the leading cause of morbidity and mortality in diabetes (6). IHD results from an imbalance between blood supply and oxygen demand in myocardial cells caused by different degrees of coronary artery obstruction, and occurs when atherosclerosis develops in the coronary artery.
Ischemia is related to inadequate oxygen supply and reduced availability of nutrients, and incomplete removal of metabolic products (7). Patients with T2DM have a higher risk of developing IHD and mortality following IHD than healthy ones (8).
Diabetes complications, such as heart diseases, develop much earlier before diagnosing (9). To prevent the progression of the disease, special attention should be paid to its early detection. Despite the rapid progress in cardiovascular research, there is no reliable tool for prompt diagnosis and identification of people at risk of developing IHD. Coronary angiography remains the gold standard in the diagnosis of IHD. Unfortunately, it is an invasive medical procedure that uses contrast dye to detect blockages in coronary arteries at x-ray pictures (10). Additionally, this method can only diagnose the disease at a later stage. Therefore, early and noninvasive detection of this condition at the initial state or diagnosis of diabetic patients with a predisposition to IHD is crucial.
Recent research shows that inflammatory mediators like chemokines CXCL12 and macrophage migration-inhibitory factor (MIF) play an essential role in the pathology of IHD. In humans, MIF is abundantly produced by various cells in different stages of plaque development (11, 12). Elevated levels of MIF in plasma can serve as an early biomarker for acute myocardial ischemia and can be risk factor for future coronary events in IHD patients with T2DM (13, 14). It has been shown that CXCL12 levels in plasma are better predictors of IHD outcomes than traditional risk factors (15, 16). Unfortunately, the serum concentration of MIF and CXCL12 seems to be characteristic not only for ischemic heart disease but also for different inflammatory states raising the need for more selective markers for IHD diagnosis (17).
A promising tool for understanding the pathogenesis of IHD is miRNAs (18). MiRNAs are small (17-25 nucleotides) non-coding RNAs that play an essential role in regulating gene expression. MiRNAs control the expression of target genes by base pairing to the 3’ untranslated regions (3’ UTRs) of mRNA and inducing repression of the target mRNA. This effect can occur by transcript destabilisation or inhibition of translation (19). Bioinformatics predictions indicate that one miRNA could target more than a hundred mRNAs (20). MiRNAs participate in critical biological processes such as proliferation, differentiation, and apoptosis of cells (21). Abnormal expression of miRNAs is associated with various diseases, such as cancer, cardiovascular diseases or metabolic disorders. It is commonly known that levels of specific circulating miRNAs might be predictive for long-term diabetes complications (22). MiRNAs may be a helpful biomarker because of their stability in biofluids even after prolonged collection and several freezing-thaw cycles (23). Moreover, those small particles can be easily collected and measured with specific, sensitive assays (22).
MiRNAs such as miR-92a, miR-503, and miR-126 might control and regulate angiogenesis, crucial for the repair of myocardial cells after ischemic injury (24, 25). MiR-155 and miR-342-5p are involved in vascular inflammation by modulation of inflammatory response and atherosclerosis progression (26). MiR-125b and miR-205 can regulate vascular calcification that contributes to atherosclerosis (27, 28). Decreased level of miR-126 is known as a predictor of diabetes, and it also occurs in patients with IHD (21). In diabetes, there is an established connection between miR-223 and activation of platelets, the latter of which significantly contributes to the development of cardiac disease (29). However, despite evident progress, our understanding of the regulation and function of specific miRNAs in IHD is still limited.
In the present study we investigated the differential expression of IHD-associated miRNAs in the serum samples from T2DM patients with and without IHD using the nCounter platform, a novel technique offering a high level of precision and sensitivity without amplification reaction (30).
Materials and Methods
Baseline Characteristics of the Patients
To diagnose IHD in over 600 patients who participated in the cohort study conducted in the Department of Invasive Cardiology, Medical University of Bialystok, coronary angiography was performed. This procedure has distinguished two subsets of T2DM patients – with IHD (n = 24) and without IHD (n = 20), IHD group (n = 9) and the control group without diabetes or prediabetes and IHD (n = 16). T2DM was confirmed according to the Diabetes Poland criteria (31). Exclusion criteria for this study included: type 1 diabetes mellitus, latent autoimmune diabetes of adults, other T2DM complications (retinopathy, neuropathy, nephropathy, peripheral artery disease, stroke, cerebrovascular disease), previous myocardial infraction, percutaneous coronary intervention, other inflammatory states (rheumatoid arthritis, systemic sclerosis), cancers, human immunodeficiency virus (HIV) or hepatitis C virus (HCV) infection, recent surgery, alcohol consumption, and smoking. A group of 69 individuals was qualified for further analysis. Serum samples were collected, centrifuged and stored at −80°C. This study was approved by the Bioethics Committee of the Medical University of Bialystok, Poland (approval numbers: R-I-002/583/2019 and APK.002.35.2021) and was performed according to the principles of the Declaration of Helsinki.
MiRNAs Isolation
The miRNeasy Serum/Plasma Advanced Kit (Qiagen, Germany) was used for RNA extraction (smaller than 1000 nucleotides) using 200 μL of serum aliquots from one patient according to the manufacturer’s instructions. The miRNA concentration was measured by The Qubit microRNA Assay Kit (Invitrogen, California, CA, United States) with the Qubit 3.0 Fluorometer.
Detection of miRNAs Profile
A total of 69 samples were prepared for nCounter miRNA expression profiling according to the manufacturer’s recommendations (NanoString Technologies, USA). A three ng of isolated microRNA were used as input material. Unique DNA tags were ligated onto the 3′ end of each mature miRNA, providing an identifier for each miRNA in the sample. Tagging was performed in the ligation reaction followed by an overnight hybridization (65°C) to nCounter Reporter and Capture probes that allowed complex sequence-specific probes with targets. After hybridization, samples were placed into the nCounter Prep Station for automated sample purification and target/probe complexes immobilization on the cartridge for data collection. Each sample was scanned for 555 FOV (fields of view) on the nCounter Digital Analyzer (NanoString Technologies, USA) to count individual fluorescent barcodes and quantify target RNA molecules present in each sample. NanoString raw data were analysed with nSolver software (NanoString Technologies, USA).
Measurement of MIF and CXCL12 in Serum
The concentration of MIF and CXCL12 in serum was determined in duplicate samples by enzyme-linked immunosorbent assay (ELISA) (Quantikine Human M-CSF Immunoassay; R&D systems, Abingdon, United Kingdom), according to the manufacturer’s recommendations.
Validation of the NanoString Results by Real-Time Quantitative Polymerase Chain Reaction (qRT-PCR)
The serum of 22 T2DM and 26 T2DM IHD miRNA samples were reverse-transcribed using a miRCURY LNA RT Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions, on a Proflex thermal cycler (Thermo Fisher Scientific, Waltham, USA). Subsequently, the qRT-PCR reaction was performed using specific primers and a miRCURY LNA SYBR Green PCR Kit (Qiagen, Germany). The samples were run on the qPCR plates in duplicate on the LightCycler 480 Real-Time PCR System (Roche, Switzerland). Based on the NormFinder (32), miR-103a-3p and miR-199b-5p were used as endogenous reference miRNAs. The primers efficiency for targets and reference miRNAs has been checked. After calculating the qPCR efficiency, relative expression levels of miRNAs were calculated using the delta-delta Ct (2–ΔΔCt) method. Ct is the threshold cycle which is a point when fluorescence reading surpasses a set baseline. This method calculates samples’ relative fold expression, using a reference miRNA as the normalizer (33).
Statistical Analysis
Based on similar previous experiments and pilot data on NanoString nCounter miRNA Expression Assay we have calculated the minimal number of samples per experimental group (T2DM IHD or T2DM) in order to detect 1.5 fold differences in relative miRNA expression level between groups at the true positives detection powers of 80% and 90% under p=0.05 (34). We have used RNASeqPower R package (35) applying the statistics data covering obtained counts and coefficients of variations per different groups. We have estimated that to obtain 80% power we would need 12 samples, whereas to obtain high power of 90% we would need 16 samples per group. Finally our groups consisted of 20 samples in T2DM and 24 in IHD T2DM group thus allowing for more than 90% power under p=0.05. Statistical analyses were performed using STATISTICA version 13.1 (StatSoft, Tulsa, Oklahoma). Preliminary statistical analysis (Shapiro–Wilk test) revealed that the studied parameters did not follow a normal distribution. The ANOVA Kruskal-Wallis test followed by Dunn’s test was performed to examine the difference in clinical parameters between the groups. miRNAs were tested for differential expression using nSolver 4.0 Analysis software (NanoString), including normalization using the positive ligation controls. The threshold count value was set to 20. The p-value was adjusted using the False Discovery Rate (FDR) correction for multiple comparisons limited to 0.05. Ingenuity Pathway Analysis (QIAGEN Inc.) was performed to generate a list of predicted targeted genes for studied miRNAs and identify canonical pathways. To find the highly connected hub genes of six miRNAs in the protein-protein network (PPI) STRING database (http://string-db.org) (36), Cytoscape version 3.9.0 (http://cytoscape.org/) (37) and its plugin (cytoHubba) were applied. GO and functional annotation clustering were carried out using the KEGG (Kyoto Encyclopedia of Genes and Genomes) Enrichment Analysis; g:Profiler (https://biit.cs.ut.ee/gprofiler/gos), and Metascape (https://metascape.org) online databases. GO analysis permits linking a list of genes to specific functional annotations categorized into functional groups (38). The Revigo web-based tool (http://revigo.irb.hr/) was used to summarise and visualise lists of Gene Ontology. The Spearman rank-order correlation coefficient (r) was determined to estimate the correlation between the identified miRNAs and clinical parameters. It was assumed that r > 0.8 indicates a strong correlation, and r > 0.3 shows a moderate correlation. The statistical significance level was set at p < 0.05. Receiver operating characteristic (ROC) analysis was used to assess the diagnostic value of miRNAs, and for each miRNA, the area under the curve (AUC) was calculated. The logistic regression model was created using Weka software to assess the diagnostic values of multi-miRNAs assays. MiRNAs for analysis were selected using attribute selection (evaluator: Info Gain, search: Ranker). MiRNAs with the highest ranks were used for further modelling. Logistic regression, random tree, J48 tree, and naive bayes classification algorithms with 10-fold cross-validation were used to select the combination of miRNAs with the highest diagnostic value.
Results
Patient Baseline Characteristic
The study groups consisted of 44 patients with T2DM; 24 of them were diagnosed with IHD. Nine patients suffered only from IHD, without T2DM. Sixteen patients were control group with normal glucose tolerance and negative coronarography results. IHD and IHD-free groups did not show significant differences in clinical parameters such as duration of diabetes, platelets, fibrinogen, body mass index (BMI), glucose level, glycated haemoglobin (HbA1c) level, blood pressure, cholesterol, triglycerides (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL). The clinical characteristics of patients enrolled in this study are summarized in Table 1. Patients in both diabetes groups had been treated with standard regimens and took oral hypoglycaemic medications. Patients with T2DM had been treated with derivatives of biguanide (42% of T2DM and 50% of T2DM IHD group, p > 0.05) and, sulfonylurea derivatives (58% of T2DM and 68% of T2DM IHD group, p > 0.05).
Table 1.
Characteristics | Patients (n = 16) controls | Patients (n = 9) IHD | Patients (n = 20) T2DM | Patients (n = 24) T2DM IHD |
---|---|---|---|---|
median (Q1-Q3) | median (Q1-Q3) | median (Q1-Q3) | median (Q1-Q3) | |
Age [years] | 52.32 (49.88 - 55.91) | 54.85 (50.57 - 61.20) | 57.50 (52.33 - 65.17) | 58.33 (53.4 - 64.58) |
T2DM duration [years] | – | – | 7.00 (4.00 -10.00) | 5.00 (2.00-10.00) |
BMI [kg/m2] | 24.62 (22.19 - 25.66) | 27.73 (26.47 - 30.39)a* | 32.19 (28.63 - 36.41)a**** | 31.24 (27.68 - 33.20)a*** |
Platelets [103/μL] | 227.50 (163.00 - 268.00) | 221.00 (195.00 - 248.00) | 222.00 (197.00 - 243.00) | 225.00 (186.00 - 322.00) |
Fibrinogen [mg/dL] | 333.50 (272.00 - 352.00) | 362.00 (296.00 - 413.00) | 352.00 (315.00 - 381.00) | 416.00 (354.00 - 482.00)a** |
Leukocytosis [103/μL] | 6.45 (5.49 - 7.30) | 7.51 (5.60 - 8.90) | 7.16 (5.40 -8.47) | 7.32 (6.38 - 9.30) |
Fasting glucose [mg/dL] | 89.00 (85.00 - 91.00) | 90.00 (84.00 - 100.00) | 122.00 (118.00 - 165.00)a****,b*** | 129.00 (104.00 - 176.00)a***,b** |
HbA1c [%] | 5.50 (5.50 - 5.65) | 5.50 (5.30 - 5.70) | 6.20 (5.70 - 7.50)a*,b* | 7.60 (6.20 - 8.80)a***,b*** |
Systolic blood pressure [mmHg] | 120.00 (115.00 - 130) | 120.00 (110.00 - 140.00) | 140.00 (120.00 – 155.00)a* | 140.00 (130.00 – 155.00)a***,b* |
Diastolic blood pressure [mmHg] | 80.00 (70.00 - 80.00) | 80.00 (80.00 - 100.00) | 80.00 (80.00 - 90.00) | 85.00 (80.00 - 90.00) |
Cholesterol [mg/dL] | 194.00 (162.00 - 209.00) | 176.00 (163.00 - 196.00) | 198.50 (172.00 - 224.00) | 179.50 (138.00 - 211.00) |
TG [mg/dL] | 109.00 (86.00 - 153.00) | 134.00 (109.00 - 183.00) | 124.00 (90.00 - 202.00) | 179.00 (109.00 - 206.00) |
LDL [mg/dL] | 112.00 (92.00 - 132.00) | 106.00 (102.00 - 117.00) | 118.50 (102.00 - 135.00) | 102.00 (66.00 - 133.00) |
HDL [mg/dL] | 49.00 (40.00-62.00) | 35.00 (33.00 - 45.00) | 46.00 (41.00 - 57.00)b* | 39.00 (34.00 - 50.00) |
Significantly different from control group;bSignificantly different from IHD group *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; CI, confidence interval; the p-value describes the significance of the difference between patients with T2DM without IHD compared to patients with T2DM and IHD using ANOVA Kruskal-Wallis test followed by Dunn’s test.
Baseline Levels of Circulating miRNAs
To determine the differentially expressed miRNAs (DEMs) in serum of diabetic patients with IHD (n = 24) compared to patients with T2DM (n = 20), patients with IHD (n = 9), and control group (n = 16), we performed the expression analysis of 798 unique miRNAs using the nCounter platform. nSolver 4.0 analysis indicated that 14 miRNAs were identified as differentially expressed between T2DM IHD and T2DM groups. All of them were upregulated in serum samples of T2DM patients with IHD when compared to diabetic patients without IHD (|FC| ≥ 1.5, FDR ≤ 0.05) (Supplementary Table 1). Altogether 155 DEMs were found between the T2DM IHD compared to the control group (Supplementary Table 2). Additionally, 155 DEMs were found between T2DM and control group (Supplementary Table 3). Furthermore, 221 DEMs were found between T2DM IHD and IHD groups (Supplementary Table 4). Those comparisons allowed extract six DEMs typical only for T2DM IHD patients (Table 2).
Table 2.
miRNA | FC | p-value | FDR |
---|---|---|---|
miR-615-3p | 2.45 | 0.00000497 | 0.00 |
miR-3147 | 2.35 | 0.00000200 | 0.00 |
miR-1224-5p | 1.68 | 0.00001305 | 0.00 |
miR-5196-3p + miR-6732-3p | 1.56 | 0.00040774 | 0.01 |
miR-548b-3p | 1.55 | 0.0002042 | 0.01 |
FC, fold change; FDR, false discovery rate, adjusted p-value; FC ≥ 1.5; FDR ≤ 0.05.
Functional Enrichment Analysis in T2DM IHD Patient Samples
The IPA analysis demonstrated 489 putative target genes for six DE miRNAs (Supplementary Table 5). To visualise connections between tested miRNAs and target genes, Cytoscape version 3.9.0 (http://cytoscape.org/) was used. The obtained network of connections is shown in Figure 1.
Ingenuity core analysis identified 36 altered canonical pathways, including the endothelin-1 signalling pathway, glucocorticoid biosynthesis, and apelin cardiomyocyte signalling pathway (Supplementary Table 6). The top 20 deregulated canonical pathways are shown in Figure 2.
STRING database (36) (https://string-db.org/) in Cytoscape tool (37) and the cytoHubba plugin (39) were used to visualise the protein-protein interaction (PPI) network of the DE miRNA target genes and identify top 10 hub genes (Figure 3)
According to cytoHubba plugin’s MCC (The Maximal Clique Centrality) ranking, the top 10 hub genes in the PPI network were C3AR1 (Complement C3a Receptor 1), CCR5 (C-C Motif Chemokine Receptor 5), APLNR (Apelin Receptor), and HNRNPC (Heterogeneous Nuclear Ribonucleoprotein C) targeted by miR-5196-3p; GNAI2 (G Protein Subunit Alpha I2) and OPRL1 (Opioid Related Nociceptin Receptor 1) targeted by miR-3147; CXCL8 (C-X-C Motif Chemokine Ligand 8) targeted by miR-6732-3p, ADORA1 (Adenosine A1 Receptor), CXCL6 (C-X-C Motif Chemokine Ligand 6), and GPSM3 (G Protein Signaling Modulator 3) targeted by miR-1224-5p (Figure 4).
Gene ontology (GO) enrichment analysis identified biological pathways and processes associated with the target genes. The GO-biological process (BP) analysis identified the most significant pathways included cell death, cell activation, cell-cell signalling, secretion, cell population proliferation, circulatory system development, tube development, regulation of response to external stimulus, negative regulation of execution phase of apoptosis, and tube morphogenesis. Transcription regulatory region nucleic acid binding, protein-containing complex binding, enzyme binding, hydrolase activity, acting on ester bonds, identical protein binding, transporter activity, receptor antagonist activity, calcium ion binding, signalling receptor regulator activity, and transcription factor binding were pointed as the main important in GO-molecular function analysis. According to the GO-cellular component analysis, target genes were mainly enriched in the membrane, non-membrane-bounded organelle, membrane-enclosed lumen, cytosol, extracellular region, cell periphery, nucleoplasm protein-containing complex, plasma membrane, and an intrinsic component of membrane. KEGG pathway enrichment analysis showed pathways involved in cardiovascular diseases and diabetes development (Figure 5).
Levels of MIF and CXCL12 in Serum
No significant differences were observed between the serum concentration of MIF and CXCL12 in T2DM IHD and T2DM groups (p>0.05). The median values of MIF were 0.65 ng/ml in the T2DM IHD group and 0.85 ng/ml in the T2DM group. The median values of CXCL12 in the T2DM IHD and T2DM groups were 2001.54 pg/ml and 2158.37 pg/ml, respectively (p > 0.05). Both levels of CXCL12 were significantly different in the T2DM and T2DM IHD compared to the control group (Supplementary Table 7). These results indicate that neither MIF nor CXCL12 can serve as good IHD prognostic markers in T2DM patients.
Correlation of Circulating miRNAs With Clinical Data
A Spearman’s rank-order regression analysis was performed to assess the relationship between miRNAs levels and clinical parameters of patients (Figure 6). The analysis indicated significant moderate correlation between fibrinogen and miR-1224-5p, miR-3147, miR-5196-3p+miR-6732-3p, and miR-615-3p (r = 0.38; r = 0.40; r = 0.41; r = 0.36 respectively; p < 0.05). Platelet count was negatively correlated with miR-548b-3p (r = -0.4; p < 0.05). Systolic blood pressure was correlated with miR-1224 (r = 0.31; p < 0.05). Analysis indicated moderate correlation between HDL and miR-615-3p (r = -0.42, p < 0.05). Statistical analysis showed strong positive correlation between serum levels of all studied miRNAs. The strongest positive correlation was indicated between miR-5196-3p+miR-6732-3p and miR-3147 (r = 0.81; p < 0.001). MiR-3147 was also correlated with miR-615 and with miR-548b-3p (r = 0.65; r = 0.60 respectively; p < 0.001). MiR-1224-5p showed correlation with miR-548b-3p, miR-5196-3p+miR-6732-3p, miR-3147 (r = 0.70; r = 0.77; r = 0.68; p < 0.001). MiR-615-3p showed correlation with miR-5196-3p+miR-6732-3p and miR-548b-3p (r = 0.72; r = 0.49 respectively; p < 0.001). No significant correlations between MIF and CXCL12 levels and upregulated miRNAs were found.
Evaluation of Diagnostic Values of Tested miRNAs
ROC curve analysis was performed to estimate the possible roles of identified miRNAs as biomarkers for IHD in diabetic patients (Figure 7). AUC (area under the ROC curve) for all six miRNAs reached significance comparing to AUC = 0.500 (p < 0.001 in all cases). The AUC values > 0.800 were found for miR-1224-5p, miR-3147, miR-5196-3p+miR-6732-3p and miR-615-3p. AUC score for the miR-548b-3p was also high (AUC = 0.779). The AUC values for MIF and CXCL12 were less than 0.500 (AUC = 0.5 borderline of the diagnostic usefulness of the test).
Logistic Regression Model
To investigate the possible increase of diagnostic value by simultaneous consideration of multiple DE miRNAs, logistic regression models were developed using Weka 3.8.6 (The University of Waikato, Hamilton, New Zealand) software (40). The parameters of the models and the standard quality measures are summarized in the Table 3. The highest AUC was obtained for a combination of miR-3147 and miR-615-3p (AUC = 0.935). This model had a higher diagnostic value compared to the highest AUC for miRNA used separately.
Table 3.
model | TP rate | FP rate | Precision | AUC | Intercept | Coefficients |
---|---|---|---|---|---|---|
x1 = miR-3147 | 0.864 | 0.147 | 0.866 | 0.935 | 28.56 | a1 = -9.03 |
x2 = miR-615-3p | a2 = -6.87 | |||||
x1 = miR-548b-3p | 0.841 | 0.166 | 0.841 | 0.929 | 20.30 | a1= -7.06 |
x2 = miR-615-3p | a2= -7.22 | |||||
x1 = miR-3147 | 0.886 | 0.120 | 0.887 | 0.927 | 26.78 | a1 = -6.49 |
x2 = 548b-3p | a2 = -3.09 | |||||
x3 = 615-3p | a3 = -6.54 | |||||
x1 = 1224-5p | 0.795 | 0.204 | 0.797 | 0.906 | 20.68 | a1 = -8.78 |
x2 = 615-3p | a2 = -5.87 | |||||
x1 = miR-3147 | 0.795 | 0.212 | 0.795 | 0.881 | 28.00 | a1 = -6.69 |
x2 = miR-615-3p | a2 = -6.47 | |||||
x3 = 1224-5p | a3 = -2.06 | |||||
x4 = 548b-3p | a4 = -2.27 | |||||
x5 = 5196-3p+miR-6732-3p | a5 = 0.55 |
TP, true positive; FP, false positive; AUC, area under the curve of the receiver operating characteristic.
Data Validation
To verify the results of the NanoString analysis, the most upregulated miRNAs from the miRNA profiling (miR-615-3p, miR-3147) were validated in the group of 22 T2DM and 26 T2DM IHD patients. In comparison with the results from the nCounter platform to qRT-PCR validation showed similarity between the expression patterns of tested miRNA (Figure 8).
Discussion
T2DM is a complex metabolic disorder involving multiple genes that affect different signalling pathways. Diabetes is a known risk factor for IHD, and patients with higher HbA1c levels are more at risk of cardiovascular mortality (41). The development of new molecular biomarkers of identification T2DM patients with risk of IHD can improve the care of such patients. MiRNAs, as essential mediators of cell-to-cell communication, have critical roles in the epigenetic regulation of metabolic, inflammatory, and antiangiogenic pathways in diabetes-related to long term complications (42). The latest research show that miRNA can play an important role as a biomarker for diabetes and its complications (3). The potential for biofluids–derived miRNA to serve as a diagnostic tool has stimulated a wide range of studies regarding the disease-specific expression of miRNA and its stability (43). To find the unique miRNA profile in T2DM patients with IHD, we used a state-of-the-art NanoString nCounter platform that provides the opportunity to profile a large number of miRNAs that have not been previously investigated about IHD in T2DM.
In comparison to other methods of miRNA detection, the NanoString offers maximal sensitivity and specificity with high-quality data due to the elimination of amplification (44). It has been shown that this platform detects miRNAs in biofluids with sensitivity and specificity more significant than other miRNA detection methods like qPCR or microarrays (45). Moreover, this technique represents a more accessible and accurate method for everyday clinical practice because of the partially automated process that eliminates the risk of errors (46). To our best knowledge, this study is the first miRNA profiling in the serum of IHD patients using the nCounter platform, which seems to be an optimal strategy to identify novel biomarker candidates for IHD prognosis.
Our research revealed six DE miRNAs in patients with T2DM and IHD compared to diabetic individuals without IHD. No significant expression of those miRNAs was observed in the group of T2DM without IHD.
Upregulated miRNAs in the T2DM IHD group with the highest fold change and AUC value were miR-615-3p and miR-3147 (FC = 2.45 and 2.35; AUC = 0.877 and 0.858 respectively). A study by Zong et al. compared miRNA profiles in patients with acute myocardial infarction to controls. The results from RNA sequencing pointed out 96 up-, and 85 down-regulated miRNAs included miR-615-3p, which was also confirmed by qPCR validation. In this case, the AUC for miR-615-3p was 0.688 (47). MiR-3147,miR-1224-5p, miR-5196-3p, miR-6732-3p, and miR-548b-3p have never been previously described concerning T2DM or vascular disease. Their indication in our study may evidence that they are new potential biomarkers of IHD in T2DM. These results suggest that indicated miRNAs candidates have a high probability of being specific for the T2DM IHD phenotype and could play a pivotal role in the IHD diagnosis in T2DM patients.
The IPA analysis indicated 489 molecules regulated by identified miRNAs. Further analysis linked the target genes into a PPI network and extracted ten hub genes. Some of them are connected with cardiovascular abnormalities and T2DM. C3aR1 showed elevated expression in obese patients compared to the control group (48). Research implicates that CCR5 plays an important role in the initiation and progression of atherosclerosis (49). CXCL6 and CXCL8 belong to the cytokine family connected with heart failure and T2DM (50, 51). ADORA1 gene encodes adenosine A1 receptor and, in the heart, it is expressed in cardiomyocytes, and its activation might promote angiogenesis (52). ADORA1 is upregulated in the epicardial adipose tissue, which may be involved in IHD pathogenesis compared with mediastinal adipose tissue (53). Adenosine signalling has a crucial role in diabetes mellitus pathophysiology due to its modulation of insulin secretion and regulation of β-cell homeostasis (54). Our analysis showed that APLNR, which encodes apelin receptor, is a target gene for miR-5196-3p. Apelin showed vascular effects in numerous studies and under normal conditions, it lowers blood pressure (55, 56). Recent studies have found that apelin-mediated signalling is connected to heart failure and IHD. Furthermore, apelin participates in the pathology of diabetes by playing a pivotal role in increasing glucose uptake and insulin sensitivity (57). Moreover, a meta-analysis study showed that circulating apelin levels in humans are higher in T2DM patients than in healthy controls (58). In contrast, a study by Castan-Laurell et al. has proven that apelin can regulate blood glucose levels, and elevated levels of plasma apelin might be beneficial in reducing the risk of diabetes (59). Given that most of the presented miRNAs have not been previously described and are potentially associated with this gene involved in critical processes leading to IHD, special attention should be paid to it in further studies.
Ingenuity core analysis allowed us to identify which canonical pathways are dysregulated by tested upregulated miRNAs. Our results showed that one of the most important dysregulated canonical pathways in the T2DM IHD patients was endothelin-1 signalling. Endothelin-1 is a potent vasoconstrictor and pro-inflammatory protein and is an important contributor to the pathogenesis of hypertension, atherosclerosis, hypertrophy, and diabetes (60). Influenced by risk factors for cardiovascular disorders, its expression is altered, which plays an important role in the pathology of the cardiovascular system (61). Furthermore, patients with T2DM have increased vasoconstrictor activity induced by endothelin-1 (62). It has been proven that p38 MPAK has a crucial role in myocyte proliferation and apoptosis in the heart and participates in cardiac hypertrophy regulation (63). Interestingly Liang et al. conducted a study that confirms that miR-124 inhibits macrophage cells by targeting the p38/MAPK signalling pathway in the development of atherosclerosis. Mineralocorticoid biosynthesis is another canonical pathway that might be involved in IHD, especially at the atherosclerosis development (64, 65). Similarly, glucocorticoids and their receptors are also related to blood pressure regulation, atherosclerosis, and heart failure (66). Another important dysregulated canonical pathway is the pentose phosphate pathway which is a significant pathway for glucose metabolism, is required to synthesise ribonucleotides and produces a reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), which is a key reductant in anabolic processes (67). This pathway participates in the T2DM pathogenesis (68). Melatonin signalling is another dysregulated canonical pathway in T2DM IHD patients. Melatonin has a considerable role in arterial blood pressure, insulin resistance, lipid and glucose metabolism, and control sleep-wake cycles. Patients with IHD have low melatonin production rates. Interestingly, this analysis also confirmed the importance of apelin signalling. Indeed, these identified canonical pathways play a significant role in the mechanisms leading to the development of cardiovascular diseases. It allows supposing that miRNAs determined by us play a relevant role in the regulation of genes associated with cardiovascular diseases and top dysregulated canonical pathways.
Nevertheless, we did not find any statistically significant correlation between the level of miRNAs and clinical parameters such as age, BMI, leucocytes, fasting glucose, HbA1c, diastolic blood pressure, cholesterol, triglycerides, or LDL level. However, those parameters are not specific only for IHD. Fibrinogen is not only an indicator of hypercoagulability but, as an acute-phase protein, is also an indicator of inflammation. In epidemiological and clinical studies, elevated blood fibrinogen levels are an independent risk factor for cardiovascular diseases (69–71). It has been proven that miRNAs can regulate fibrinogen production (72). Interestingly, we indicated a moderate positive correlation between levels of fibrinogen and miR-1224-5p, miR-3147, miR-5196-3p+miR-6732-3p, and miR-615-3p which have not been previously described as being related to this protein. We have indicated a moderate negative correlation between miR-548b-3p and platelets. The link between miRNAs and platelet in the development of IHD has been previously proven and summed up in a review by Stojkovic et al. (73). A strong or moderate positive correlation between all miRNA’s levels suggested they belong to the miRNA group, which dysregulation of mechanisms responsible for diabetic complications.
The area under the ROC curve remains a major criterion for diagnostic biomarkers. The ROC curve illustrates the relationship between diagnostic sensitivity and specificity and describes the diagnostic value of the tested parameters (74). To the best of our knowledge, there is no evidence in the literature describing the usefulness of miR-615-3p, miR-3147, (miR-1224-5p, miR-5196-3p+miR-6732-3p, miR-548b-3p) as a diagnostic tool for IHD in T2DM. The highest AUC values were observed in this study for miR-615-3p and miR-3147. Furthermore, other tested miRNA (miR-1224-5p, miR-5196-3p+miR-6732-3p, and miR-548b-3p) also showed high AUC results. The combined analysis showed that a logistic regression model consisting of miR-3147 and miR-615-3p demonstrates higher diagnostic accuracy than those miRNAs individually. These findings suggest that it would be beneficial to introduce such a panel in predicting IHD development in T2DM patients. High AUC scores for these miRNAs provide the groundwork for future confirmatory studies with a comprehensive validation in a larger cohort of patients. Additionally, AUC values for MIF and CXCL12, which play an important role in IHD, were below 0.500, disqualifying these two proteins as a good diagnostic tool for IHD diabetic patients.
MIF and CXCL12 are considered as a potential biomarker for heart diseases in patients with T2DM (75). However, one must consider that diabetes is also an inflammation state, and non-specific inflammatory parameters like MIF or CXCL12 can be elevated in patients with IHD or without it. Low AUC values for those proteins support our observations that the changes in miRNA levels could be better prognostic IHD biomarkers than the level of MIF or CXCL12 in T2DM patients.
Currently, the RT-qPCR technique, which we have used to validate results, remains the most commonly used approach for miRNA expression profiling due to its price, sensitivity, and specificity. The RT-qPCR analysis showed significantly increased expressions of miR-615-3p and miR-3147 in the serum of patients with T2DM who developed IHD, which confirms the reliability of the nCounter platform.
Presented data indicate a specific serum miRNA profile of patients with T2DM and IHD, and the different levels of selective miRNA expression might have a crucial role in further IHD diagnosis. We believe that identified circulating miRNAs might serve as new, non-invasive biomarkers for early detection of IHD in T2DM patients. However, this data should be considered preliminary. Additional studies on a larger cohort of patients are required to validate the predictive value of those miRNAs to prompt diagnosis of IHD in T2DM patients or better identification of at-risk individuals.
Data Availability Statement
The original contributions presented in the study are publicly available. This data can be found below: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE185845
Ethics Statement
The studies involving human participants were reviewed and approved by The Bioethics Committee of the Medical University of Bialystok, Poland (approval numbers: R-I-002/583/2019 and APK.002.35.2021). The patients/participants provided their written informed consent to participate in this study.
Author Contributions
Conceptualization, AB, MN, and AK; methodology, AB, MN, IS, ASz, and AK; formal analysis, AB, MN, IS, and WB, investigation, AB, ASz, JR, DO, KG, and MN; writing—original draft preparation, AB; writing—review and editing, AK, MN, and ASk; visualization, AB and MN; supervision, MN, SD, and AK. All authors have read and agreed to the published version of the manuscript.
Funding
This study was funded by internal financing of the Medical University of Bialystok SUB/1/NN/21/001/4406 and was supported by the funds of the Ministry of Education and Science within the project “Excellence Initiative - Research University”.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Acknowledgments
The first version of this manuscript was published as a preprint with digital object identifier DOI number 10.21203/rs.3.rs-42388/v (76).
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fendo.2022.888948/full#supplementary-material
References
- 1. Saeedi P, Salpea P, Karuranga S, Petersohn I, Malanda B, Gregg EW, et al. Mortality Attributable to Diabetes in 20-79 Years Old Adults, 2019 Estimates: Results From the International Diabetes Federation Diabetes Atlas, 9(Th) Edition. Diabetes Res Clin Pract (2020) 162:108086. doi: 10.1016/j.diabres.2020.108086 [DOI] [PubMed] [Google Scholar]
- 2. Mao J, Ai J, Zhou X, Shenwu M, Ong M, Jr., Blue M, et al. Transcriptomic Profiles of Peripheral White Blood Cells in Type II Diabetes and Racial Differences in Expression Profiles. BMC Genomics (2011) 12 (Suppl 5):S12. doi: 10.1186/1471-2164-12-S5-S12 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3. Moura J, Borsheim E, Carvalho E. The Role of Micrornas in Diabetic Complications-Special Emphasis on Wound Healing. Genes (Basel) (2014) 5(4):926–56. doi: 10.3390/genes5040926 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4. Bielska A, Niemira M, Kretowski A. Recent Highlights of Research on Mirnas as Early Potential Biomarkers for Cardiovascular Complications of Type 2 Diabetes Mellitus. Int J Mol Sci (2021) 22(6):3153. doi: 10.3390/ijms22063153 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5. Miura M, Sakata Y, Miyata S, Nochioka K, Takada T, Tadaki S, et al. Prognostic Impact of Diabetes Mellitus in Chronic Heart Failure According to Presence of Ischemic Heart Disease - With Special Reference to Nephropathy. Circ J (2015) 79(8):1764–72. doi: 10.1253/circj.CJ-15-0096 [DOI] [PubMed] [Google Scholar]
- 6. Hartz JC, de Ferranti S, Gidding S. Hypertriglyceridemia in Diabetes Mellitus: Implications for Pediatric Care. J Endocr Soc (2018) 2(6):497–512. doi: 10.1210/js.2018-00079 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7. Hausenloy DJ, Yellon DM. Myocardial Ischemia-Reperfusion Injury: A Neglected Therapeutic Target. J Clin Invest (2013) 123(1):92–100. doi: 10.1172/JCI62874 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 8. Kapur A, De Palma R. Mortality After Myocardial Infarction in Patients With Diabetes Mellitus. Heart (2007) 93(12):1504–6. doi: 10.1136/hrt.2006.112656 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9. Rawal S, Manning P, Katare R. Cardiovascular Micrornas: As Modulators and Diagnostic Biomarkers of Diabetic Heart Disease. Cardiovasc Diabetol (2014) 13:44. doi: 10.1186/1475-2840-13-44 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10. Stefanini GG, Windecker S. Can Coronary Computed Tomography Angiography Replace Invasive Angiography? Coronary Computed Tomography Angiography Cannot Replace Invasive Angiography. Circulation (2015) 131(4):418–25; discussion 26. doi: 10.1161/CIRCULATIONAHA.114.008148 [DOI] [PubMed] [Google Scholar]
- 11. Burger-Kentischer A, Goebel H, Seiler R, Fraedrich G, Schaefer HE, Dimmeler S, et al. Expression of Macrophage Migration Inhibitory Factor in Different Stages of Human Atherosclerosis. Circulation (2002) 105(13):1561–6. doi: 10.1161/01.cir.0000012942.49244.82 [DOI] [PubMed] [Google Scholar]
- 12. van der Vorst EP, Doring Y, Weber C. Mif and Cxcl12 in Cardiovascular Diseases: Functional Differences and Similarities. Front Immunol (2015) 6:373. doi: 10.3389/fimmu.2015.00373 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13. Fan F, Fang L, Moore XL, Xie X, Du XJ, White DA, et al. Plasma Macrophage Migration Inhibitor Factor Is Elevated in Response to Myocardial Ischemia. J Am Heart Assoc (2016) 5(7):3128. doi: 10.1161/JAHA.115.003128 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 14. Makino A, Nakamura T, Hirano M, Kitta Y, Sano K, Kobayashi T, et al. High Plasma Levels of Macrophage Migration Inhibitory Factor Are Associated With Adverse Long-Term Outcome in Patients With Stable Coronary Artery Disease and Impaired Glucose Tolerance or Type 2 Diabetes Mellitus. Atherosclerosis (2010) 213(2):573–8. doi: 10.1016/j.atherosclerosis.2010.09.004 [DOI] [PubMed] [Google Scholar]
- 15. Sjaarda J, Gerstein H, Chong M, Yusuf S, Meyre D, Anand SS, et al. Blood Csf1 and Cxcl12 as Causal Mediators of Coronary Artery Disease. J Am Coll Cardiol (2018) 72(3):300–10. doi: 10.1016/j.jacc.2018.04.067 [DOI] [PubMed] [Google Scholar]
- 16. Ghasemzadeh N, Hritani AW, De Staercke C, Eapen DJ, Veledar E, Al Kassem H, et al. Plasma Stromal Cell-Derived Factor 1alpha/Cxcl12 Level Predicts Long-Term Adverse Cardiovascular Outcomes in Patients With Coronary Artery Disease. Atherosclerosis (2015) 238(1):113–8. doi: 10.1016/j.atherosclerosis.2014.10.094 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 17. Dotan I, Werner L, Vigodman S, Weiss S, Brazowski E, Maharshak N, et al. Cxcl12 Is a Constitutive and Inflammatory Chemokine in the Intestinal Immune System. Inflammation Bowel Dis (2010) 16(4):583–92. doi: 10.1002/ibd.21106 [DOI] [PubMed] [Google Scholar]
- 18. Bronze-da-Rocha E. Micrornas Expression Profiles in Cardiovascular Diseases. BioMed Res Int (2014) 2014:985408. doi: 10.1155/2014/985408 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19. Flowers E, Froelicher ES, Aouizerat BE. Microrna Regulation of Lipid Metabolism. Metabolism (2013) 62(1):12–20. doi: 10.1016/j.metabol.2012.04.009 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 20. Friedman RC, Farh KK, Burge CB, Bartel DP. Most Mammalian Mrnas Are Conserved Targets of Micrornas. Genome Res (2009) 19(1):92–105. doi: 10.1101/gr.082701.108 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 21. Ding Y, Sun X, Shan PF. Micrornas and Cardiovascular Disease in Diabetes Mellitus. BioMed Res Int (2017) 2017:4080364. doi: 10.1155/2017/4080364 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 22. Guay C, Regazzi R. Circulating Micrornas as Novel Biomarkers for Diabetes Mellitus. Nat Rev Endocrinol (2013) 9(9):513–21. doi: 10.1038/nrendo.2013.86 [DOI] [PubMed] [Google Scholar]
- 23. Lima J, Jr., Batty JA, Sinclair H, Kunadian V. Micrornas in Ischemic Heart Disease: From Pathophysiology to Potential Clinical Applications. Cardiol Rev (2017) 25(3):117–25. doi: 10.1097/CRD.0000000000000114 [DOI] [PubMed] [Google Scholar]
- 24. Bonauer A, Carmona G, Iwasaki M, Mione M, Koyanagi M, Fischer A, et al. Microrna-92a Controls Angiogenesis and Functional Recovery of Ischemic Tissues in Mice. Science (2009) 324(5935):1710–3. doi: 10.1126/science.1174381 [DOI] [PubMed] [Google Scholar]
- 25. Mocharla P, Briand S, Giannotti G, Dorries C, Jakob P, Paneni F, et al. Angiomir-126 Expression and Secretion From Circulating Cd34(+) and Cd14(+) Pbmcs: Role for Proangiogenic Effects and Alterations in Type 2 Diabetics. Blood (2013) 121(1):226–36. doi: 10.1182/blood-2012-01-407106 [DOI] [PubMed] [Google Scholar]
- 26. Nazari-Jahantigh M, Wei Y, Noels H, Akhtar S, Zhou Z, Koenen RR, et al. Microrna-155 Promotes Atherosclerosis by Repressing Bcl6 in Macrophages. J Clin Invest (2012) 122(11):4190–202. doi: 10.1172/JCI61716 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 27. Qiao W, Chen L, Zhang M. Microrna-205 Regulates the Calcification and Osteoblastic Differentiation of Vascular Smooth Muscle Cells. Cell Physiol Biochem (2014) 33(6):1945–53. doi: 10.1159/000362971 [DOI] [PubMed] [Google Scholar]
- 28. Goettsch C, Rauner M, Pacyna N, Hempel U, Bornstein SR, Hofbauer LC. Mir-125b Regulates Calcification of Vascular Smooth Muscle Cells. Am J Pathol (2011) 179(4):1594–600. doi: 10.1016/j.ajpath.2011.06.016 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 29. Elgheznawy A, Shi L, Hu J, Wittig I, Laban H, Pircher J, et al. Dicer Cleavage by Calpain Determines Platelet Microrna Levels and Function in Diabetes. Circ Res (2015) 117(2):157–65. doi: 10.1161/CIRCRESAHA.117.305784 [DOI] [PubMed] [Google Scholar]
- 30. Tsang HF, Xue VW, Koh SP, Chiu YM, Ng LP, Wong SC. Nanostring, a Novel Digital Color-Coded Barcode Technology: Current and Future Applications in Molecular Diagnostics. Expert Rev Mol Diagn (2017) 17(1):95–103. doi: 10.1080/14737159.2017.1268533 [DOI] [PubMed] [Google Scholar]
- 31. Araszkiewicz A, Bandurska-Stankiewicz E, Budzyński A, Cypryk K, Czech A, Czupryniak L, et al. 2019 Guidelines on the Management of Diabetic Patients. A Position Diabetes Poland Clin Diabetol (2019) 8(1):1–95. doi: 10.5603/dk.2019.0001 [DOI] [Google Scholar]
- 32. Andersen CL, Jensen JL, Orntoft TF. Normalization of Real-Time Quantitative Reverse Transcription-Pcr Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets. Cancer Res (2004) 64(15):5245–50. doi: 10.1158/0008-5472.CAN-04-0496 [DOI] [PubMed] [Google Scholar]
- 33. Livak KJ, Schmittgen TD. Analysis of Relative Gene Expression Data Using Real-Time Quantitative Pcr and the 2(-Delta Delta C(T)) Method. Methods (2001) 25(4):402–8. doi: 10.1006/meth.2001.1262 [DOI] [PubMed] [Google Scholar]
- 34. Hart SN, Therneau TM, Zhang Y, Poland GA, Kocher JP. Calculating Sample Size Estimates for Rna Sequencing Data. J Comput Biol (2013) 20(12):970–8. doi: 10.1089/cmb.2012.0283 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 35. Therneau T HS, Kocher J. Calculating Samplessize Estimates for RNA Seq Studies. R Package Version 1.34.0. (2021). [Google Scholar]
- 36. Szklarczyk D, Gable AL, Nastou KC, Lyon D, Kirsch R, Pyysalo S, et al. The String Database in 2021: Customizable Protein-Protein Networks, and Functional Characterization of User-Uploaded Gene/Measurement Sets. Nucleic Acids Res (2021) 49(D1):D605–D12. doi: 10.1093/nar/gkaa1074 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 37. Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, et al. Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction Networks. Genome Res (2003) 13(11):2498–504. doi: 10.1101/gr.1239303 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 38. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, et al. Gene Ontology: Tool for the Unification of Biology. The Gene Ontology Consortium. Nat Genet (2000) 25(1):25–9. doi: 10.1038/75556 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 39. Chin CH, Chen SH, Wu HH, Ho CW, Ko MT, Lin CY. Cytohubba: Identifying Hub Objects and Sub-Networks From Complex Interactome. BMC Syst Biol (2014) 8 Suppl 4:S11. doi: 10.1186/1752-0509-8-S4-S11 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 40. Hall M, Frank E, Holmes G, Pfahringer B, Reutemann P, Witten IH. The Weka Data Mining Software: An Update. ACM SIGKDD Explor Newslett (2009) 11(1):10–8. doi: 10.1145/1656274.1656278 [DOI] [Google Scholar]
- 41. Cavero-Redondo I, Peleteiro B, Alvarez-Bueno C, Rodriguez-Artalejo F, Martinez-Vizcaino V. Glycated Haemoglobin A1c as a Risk Factor of Cardiovascular Outcomes and All-Cause Mortality in Diabetic and Non-Diabetic Populations: A Systematic Review and Meta-Analysis. BMJ Open (2017) 7(7):e015949. doi: 10.1136/bmjopen-2017-015949 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 42. Forbes JM, Cooper ME. Mechanisms of Diabetic Complications. Physiol Rev (2013) 93(1):137–88. doi: 10.1152/physrev.00045.2011 [DOI] [PubMed] [Google Scholar]
- 43. Pogribny IP. Micrornas as Biomarkers for Clinical Studies. Exp Biol Med (Maywood) (2018) 243(3):283–90. doi: 10.1177/1535370217731291 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 44. Veldman-Jones MH, Brant R, Rooney C, Geh C, Emery H, Harbron CG, et al. Evaluating Robustness and Sensitivity of the Nanostring Technologies Ncounter Platform to Enable Multiplexed Gene Expression Analysis of Clinical Samples. Cancer Res (2015) 75(13):2587–93. doi: 10.1158/0008-5472.CAN-15-0262 [DOI] [PubMed] [Google Scholar]
- 45. Oikonomopoulos A, Polytarchou C, Joshi S, Hommes DW, Iliopoulos D. Identification of Circulating Microrna Signatures in Crohn's Disease Using the Nanostring Ncounter Technology. Inflammation Bowel Dis (2016) 22(9):2063–9. doi: 10.1097/MIB.0000000000000883 [DOI] [PubMed] [Google Scholar]
- 46. Pescarmona R, Belot A, Villard M, Besson L, Lopez J, Mosnier I, et al. Comparison of Rt-Qpcr and Nanostring in the Measurement of Blood Interferon Response for the Diagnosis of Type I Interferonopathies. Cytokine (2019) 113:446–52. doi: 10.1016/j.cyto.2018.10.023 [DOI] [PubMed] [Google Scholar]
- 47. Zhong Z, Wu H, Zhong W, Zhang Q, Yu Z. Expression Profiling and Bioinformatics Analysis of Circulating Micrornas in Patients With Acute Myocardial Infarction. J Clin Lab Anal (2020) 34(3):e23099. doi: 10.1002/jcla.23099 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 48. Koc G, Soyocak A, Alis H, Kankaya B, Kanigur G. Changes in Vgf and C3ar1 Gene Expression in Human Adipose Tissue in Obesity. Mol Biol Rep (2021) 48(1):251–7. doi: 10.1007/s11033-020-06043-9 [DOI] [PubMed] [Google Scholar]
- 49. Jones KL, Maguire JJ, Davenport AP. Chemokine Receptor Ccr5: From Aids to Atherosclerosis. Br J Pharmacol (2011) 162(7):1453–69. doi: 10.1111/j.1476-5381.2010.01147.x [DOI] [PMC free article] [PubMed] [Google Scholar]
- 50. Hanna A, Frangogiannis NG. Inflammatory Cytokines and Chemokines as Therapeutic Targets in Heart Failure. Cardiovasc Drugs Ther (2020) 34(6):849–63. doi: 10.1007/s10557-020-07071-0 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 51. Boyko VV, Riga AS. Type 2 Diabetes Mellitus - Il-8 and Il-10 Profile in Patients With Intraabdominal Postoperative Abscesses. Wiad Lek (2020) 73(2):220–3. doi: 10.36740/WLek202002102 [DOI] [PubMed] [Google Scholar]
- 52. Dinh W, Albrecht-Kupper B, Gheorghiade M, Voors AA, van der Laan M. Sabbah HN. Partial Adenosine A1 Agonist in Heart Failure. Handb Exp Pharmacol (2017) 243:177–203. doi: 10.1007/164_2016_83 [DOI] [PubMed] [Google Scholar]
- 53. Guauque-Olarte S, Gaudreault N, Piche ME, Fournier D, Mauriege P, Mathieu P, et al. The Transcriptome of Human Epicardial, Mediastinal and Subcutaneous Adipose Tissues in Men With Coronary Artery Disease. PloS One (2011) 6(5):e19908. doi: 10.1371/journal.pone.0019908 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 54. Antonioli L, Blandizzi C, Csoka B, Pacher P, Hasko G. Adenosine Signalling in Diabetes Mellitus–Pathophysiology and Therapeutic Considerations. Nat Rev Endocrinol (2015) 11(4):228–41. doi: 10.1038/nrendo.2015.10 [DOI] [PubMed] [Google Scholar]
- 55. Mughal A, O'Rourke ST. Vascular Effects of Apelin: Mechanisms and Therapeutic Potential. Pharmacol Ther (2018) 190:139–47. doi: 10.1016/j.pharmthera.2018.05.013 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 56. Tatemoto K, Takayama K, Zou MX, Kumaki I, Zhang W, Kumano K, et al. The Novel Peptide Apelin Lowers Blood Pressure via a Nitric Oxide-Dependent Mechanism. Regul Pept (2001) 99(2-3):87–92. doi: 10.1016/s0167-0115(01)00236-1 [DOI] [PubMed] [Google Scholar]
- 57. Wysocka MB, Pietraszek-Gremplewicz K, Nowak D. The Role of Apelin in Cardiovascular Diseases, Obesity and Cancer. Front Physiol (2018) 9:557. doi: 10.3389/fphys.2018.00557 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 58. Noori-Zadeh A, Bakhtiyari S, Khanjari S, Haghani K, Darabi S. Elevated Blood Apelin Levels in Type 2 Diabetes Mellitus: A Systematic Review and Meta-Analysis. Diabetes Res Clin Pract (2019) 148:43–53. doi: 10.1016/j.diabres.2018.12.012 [DOI] [PubMed] [Google Scholar]
- 59. Castan-Laurell I, El Boustany R, Pereira O, Potier L, Marre M, Fumeron F, et al. Plasma Apelin and Risk of Type 2 Diabetes in a Cohort From the Community. Diabetes Care (2020) 43(2):e15–e6. doi: 10.2337/dc19-1865 [DOI] [PubMed] [Google Scholar]
- 60. Sandoval YH, Atef ME, Levesque LO, Li Y, Anand-Srivastava MB. Endothelin-1 Signaling in Vascular Physiology and Pathophysiology. Curr Vasc Pharmacol (2014) 12(2):202–14. doi: 10.2174/1570161112666140226122054 [DOI] [PubMed] [Google Scholar]
- 61. Bohm F, Pernow J. The Importance of Endothelin-1 for Vascular Dysfunction in Cardiovascular Disease. Cardiovasc Res (2007) 76(1):8–18. doi: 10.1016/j.cardiores.2007.06.004 [DOI] [PubMed] [Google Scholar]
- 62. Cardillo C, Campia U, Bryant MB, Panza JA. Increased Activity of Endogenous Endothelin in Patients With Type Ii Diabetes Mellitus. Circulation (2002) 106(14):1783–7. doi: 10.1161/01.cir.0000032260.01569.64 [DOI] [PubMed] [Google Scholar]
- 63. Yokota T, Wang Y. P38 Map Kinases in the Heart. Gene (2016) 575(2 Pt 2):369–76. doi: 10.1016/j.gene.2015.09.030 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 64. Moss ME, Jaffe IZ. Mineralocorticoid Receptors in the Pathophysiology of Vascular Inflammation and Atherosclerosis. Front Endocrinol (Lausanne) (2015) 6:153. doi: 10.3389/fendo.2015.00153 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 65. Zwadlo C, Bauersachs J. Mineralocorticoid Receptor Antagonists for Therapy of Coronary Artery Disease and Related Complications. Curr Opin Pharmacol (2013) 13(2):280–6. doi: 10.1016/j.coph.2012.12.007 [DOI] [PubMed] [Google Scholar]
- 66. Liu B, Zhang TN, Knight JK, Goodwin JE. The Glucocorticoid Receptor in Cardiovascular Health and Disease. Cells (2019) 8(10):1227. doi: 10.3390/cells8101227 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 67. Patra KC, Hay N. The Pentose Phosphate Pathway and Cancer. Trends Biochem Sci (2014) 39(8):347–54. doi: 10.1016/j.tibs.2014.06.005 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 68. Ge T, Yang J, Zhou S, Wang Y, Li Y, Tong X. The Role of the Pentose Phosphate Pathway in Diabetes and Cancer. Front Endocrinol (Lausanne) (2020) 11:365. doi: 10.3389/fendo.2020.00365 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 69. Wieczor R, Wieczor AM, Kulwas A, Rosc D. Type 2 Diabetes and Cardiovascular Factors Contrasted With Fibrinolysis Disorders in the Blood of Patients With Peripheral Arterial Disease. Medicina (Kaunas) (2019) 55(7):395. doi: 10.3390/medicina55070395 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 70. Fowkes FG. Fibrinogen and Cardiovascular Disease in Clinical Practice. Eur Heart J (1995) 16 Suppl:A:60–3. doi: 10.1093/eurheartj/16.suppl_a.60 [DOI] [PubMed] [Google Scholar]
- 71. Vinereanu D. Risk Factors for Atherosclerotic Disease: Present and Future. Herz (2006) 31 Suppl 3:5–24. [PubMed] [Google Scholar]
- 72. Fort A, Borel C, Migliavacca E, Antonarakis SE, Fish RJ, Neerman-Arbez M. Regulation of Fibrinogen Production by Micrornas. Blood (2010) 116(14):2608–15. doi: 10.1182/blood-2010-02-268011 [DOI] [PubMed] [Google Scholar]
- 73. Stojkovic S, Nossent AY, Haller P, Jager B, Vargas KG, Wojta J, et al. Micrornas as Regulators and Biomarkers of Platelet Function and Activity in Coronary Artery Disease. Thromb Haemost (2019) 119(10):1563–72. doi: 10.1055/s-0039-1693702 [DOI] [PubMed] [Google Scholar]
- 74. Simundic AM. Measures of Diagnostic Accuracy: Basic Definitions. EJIFCC (2009) 19(4):203–11. [PMC free article] [PubMed] [Google Scholar]
- 75. Yu H, Wang X, Deng X, Zhang Y, Gao W. Correlation Between Plasma Macrophage Migration Inhibitory Factor Levels and Long-Term Prognosis in Patients With Acute Myocardial Infarction Complicated With Diabetes. Mediators Inflammation (2019) 2019:8276180. doi: 10.1155/2019/8276180 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 76. Bielska A, Bauer W, Szalkowska A, Sidorkiewicz I, Skwarska A, Raczkowska J, et al. Serum Mirna Profile in Diabetic Patients With Ischemic Heart Disease as a Promising Noninvasive Biomarker. Res Square (2020). doi: 10.21203/rs.3.rs-42388/v1 [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Data Availability Statement
The original contributions presented in the study are publicly available. This data can be found below: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE185845